Studies on the antioxidative mode of action of phytogenic feed additives in rainbow trout

In previous trials, two phytogenic feed additives were investigated for their impact on the antioxidant status In previous trials [1], two phytogenic feed additives were investigated for their impact on the antioxidant status of rainbow trout fillets. One of the additives was rich in carvacrol (12 g/kg), while the other was rich in thymol (6 g/kg). In order to measure the antioxidant status, levels of glutathione reductase, glutathione- S-transferase, and malondialdehyde were assayed at day 0 and day 5 after slaughter. Phytogenic supplementations of rainbow trout feed improved the antioxidant status of fillets. Levels of malondialdehyde were significantly lower on day 5 and levels of glutathione-based enzymes were significantly higher at both time points compared to the control group. These findings gave rise to additional studies on antioxidative effects of phytogenics in rainbow trout to enhance our understanding of modes of action on the animal physiology. Primary cell cultures of intestinal epithelial cells were chosen as a model of the intestinal barrier which is crucial for animal health and growth. 25 fish were sampled within 5 months and a method to isolate primary gut epithelial cells ([Fig. 1]) from different intestinal segments (pyloric caeca, mid and hind gut) was successfully established. The pyloric caeca are part of the mid gut and consist of a variable number of tubes that increase the gut surface area. In the current study, the pyloric caeca showed the highest success rate for isolation of growing cell cultures and will be used for the upcoming investigations into the mode of action of phytogenics to counteract oxidative effects induced by radical oxygen species.

Fig. 1  Primary epithelial cells isolated from the pyloric caeca of a rainbow trout on day 2.

 

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• References

• 1 Giannenas I, Triantafillou El, Stavrakakis S, Margaronj M, Mavridis, Steiner T, Karagouni Assessment of dietary supplementation with carvacrol or thymol containing feed additives on performance, intestinal microbiota and antioxidant status of rainbow trout (Oncorhynchus mykiss). Aquaculture 2012; 350-353: 26-32

Publication History

Article published online:
16 November 2023