Rosmarinic acid and chlorogenic acid as gut contractility modifier in swine – ex vivo study

Rosmarinic acid and chlorogenic acid are the main active substances of Melissa officinalis which is a very common herb with high palatability. It also has a long history of being used in traditional medicine. This study aimed to develop knowledge about Melissa extract and its main active substances with regards to swine jejunum contractility.

The experiments were conducted on longitudinal and circular jejunum samples collected from routinely slaughtered swine. The effect of rosmarinic [1] and chlorogenic [2] acid on spontaneous and ACh-induced activity was evaluated under isometric conditions.

The results revealed dose-dependent potency of chlorogenic acid to increase the magnitude of acetylcholine-induced and spontaneous contractions. Rosmarinic acid worked inconsistently. This phenolic acid increased the spontaneous activity of the circular jejunum muscle but did not significantly affect the spontaneous activity of longitudinal muscles. The strength of acetylcholine-induced contraction on circular muscle decreased gradually up to the highest concentration, where it suddenly increased significantly. In the longitudinal muscularis, the force of contraction decreased after adding rosmarinic acid, but only at higher doses.

The results of the performed study indicate that rosmarinic and especially chlorogenic acid can be used to control gastrointestinal motility of swine.

 

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• References

• 1 Bazylko A, Zygmunt M., apa J, Strzelecka H., Filipek B.. Determination of spasmolytic and antispasmodic activities of thyme extracts and one of their major components, rosmarinic acid, in isolated rabbit ileum and isolated rat aorta Acta Biologica Cracoviensia . Series Zoologia 2009; 51: 49-54
• 2 Berman S.E., Huston J.L., Lail A.W., Piquette N.B., Njeti E.R.. Chlorogenic Acid lncreases Basal Tone with No Effect on Relaxation of Rat lleal Smooth Muscles by Mechanisms independent of PKG Mediated Effects on Myosin Light Chain Phosphorylation. The FASEB Journal 2019; 33 (S1): 812-9

Publication History

Article published online:
16 November 2023