Polyphenolic Compounds in Fruits and Leaves of Hippophae rhamnoides

Fruits, seeds, and leaves of Hippophae rhamnoides L. (Elaeagnaceae) are widely used in folk medicine. Although the fruits are usually used, the leaves also need attention.

The aim of the study was to estimate the content of polyphenolic compounds in methanolic and water extracts of fresh fruits and fresh and dried leaves of H. rhamnoides using LC-MS/MS method.

Almost 2.5 times more total phenols and 1.7 times more total flavonols were found in methanolic extracts (24 h) of leaves than in their water extracts (10 min). The methanolic and water extracts contained almost 10 times more total phenolics from leaves than from fruits. Methanolic and water extracts from leaves contained also more flavonols than water extracts (1.7 and 24 times, respectively).

A total of 30 compounds were found in fruits and leaves of H. rhamnoides. The methanolic and water extracts of fruits contained significant amounts of isorhamnetin rutinoside (48.5 mg/100 g and 44.4 mg/100 g, respectively), isorhamnetin glycoside (21.6 mg/100 g and 23.3 mg/100 g) and quercetin rutinoside (both about 20 mg/100 g). The methanolic extract of dried leaves contained the most catechin dimer, which was higher in fresh leaves (36 and 55 mg/100 g). The dried leaves also contained more quercetin-3-O-glucoside- 7-O-rhamnoside and its isomer than the fresh sample. Methanol extracted significantly more isorhamnetin rutinoside, isorhamnetin glucoside and isorhamnetin 3-O-glucoside-7-O-rhamnosides than water (~10, 5, and 4 times, respectively).

The leaves of H. rhamnoides deserve further attention due to their rich phenolic composition. This work was supported by the MSCA4Ukraine (1232466).

 

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Artikel online veröffentlicht:
16. November 2023