Investigation of different Tripleurospermum taxa from Greece: comparison by GC-MS and LC-HRMS

The genus Tripleurospermum (L.) Sch.Bip, Asteraceae, comprises 30 to 40 species and is distributed in the Northern Hemisphere, mainly in North America, Europe, temperate Asia and North Africa. In Greece, 7 taxa have been reported, with some of them only encountered in specific areas of the country. The current work describes the phytochemical investigation, using modern analytical techniques, of T. rosellum collected from two localities in Greece (Lesvos and Mt. Parnon) and T. tempskyanum [1]. GC-MS was applied for the analysis of the nonpolar extracts and the essential oils, revealing significant similarities between the major constituents, specifically their high concentration in matricaria easter. Digging further into the variation and distribution of minor volatile compounds, HCA analysis disclosed distinct compound-biomarkers attributed to each taxon. On the other hand, medium and high polarity extracts were analysed by means of UPLC- Orbitrap-HRMS/MS, whereby 290 compounds were determined and more than 200 tentatively identified, belonging mainly to the classes of hydroxycinnamates, flavonoids and fatty acids. Consequently, the data were subjected to multivariate analysis (MVA), to underline marker compounds distinct to each taxon. Despite the evident similarities in the extracts’ phytochemical profiles, both qualitative and quantitative differences were observed for the studied species and localities.

Funding ERDF, “RESEARCH–CREATE–INNOVATE”, CosmAGE (project code Τ2ΕΔΚ-02583)

 

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Conflict of Interest

The authors declare no conflict of interest.

• References

• 1 Oberprieler C. et al. Willdenowia 2007; 37: 89

Publication History

Article published online:
16 November 2023