Planta Med 2023; 89(14): 1369
DOI: 10.1055/s-0043-1774095
Abstracts
Tuesday 4th July 2023 | Poster Session II
Phytochemistry II - Antimicrobials; Cosmetics; Essential oils; Nutraceuticals; Biotechnology

Evaluation of the skin beneficial properties of Centaurea species belonging to the Greek flora: phytochemical investigation of Centaurea salonitana

Christina Koufali
1   Laboratory of Pharmacognosy and Natural Products Chemistry, Faculty of Pharmacy, National and Kapodistrian University of Athens, Panepistimiopolis Zografou, 15771, Athens, Greece
,
Antigoni Cheilari
1   Laboratory of Pharmacognosy and Natural Products Chemistry, Faculty of Pharmacy, National and Kapodistrian University of Athens, Panepistimiopolis Zografou, 15771, Athens, Greece
,
Nektarios Aligiannis
1   Laboratory of Pharmacognosy and Natural Products Chemistry, Faculty of Pharmacy, National and Kapodistrian University of Athens, Panepistimiopolis Zografou, 15771, Athens, Greece
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Plants of the genus Centaurea, one of the biggest genera of the Asteraceae family, have been used traditionally for hundreds of years as diuretics, to treat fever, diabetes, stomachic disorders as well as for their anti-inflammatory, antibacterial and wound healing properties [1]. The aim of the present study was to explore the phytochemical profile of Centaurea species belonging to the Greek flora, to evaluate their antioxidant properties and their ability to inhibit the enzymes tyrosinase and collagenase. Particularly, seven Centaurea species were successively extracted (dichloromethane, methanol, methanol:water, 1:1) by ultrasound-assisted extraction and the phytochemical profile of extracts was characterized by high- performance thin-layer chromatography (HPTLC), high-performance liquid chromatography (HPLC-DAD) and nuclear magnetic resonance (NMR) spectroscopy. Moreover, the total phenolic (TPC) and total flavonoid (TFC) content was determined. Finally, the DPPH scavenging capacity as well as the anti-tyrosinase and anti- collagenase activity of extracts was evaluated. The methanolic extract of Centaurea salonitana was selected for further investigation as it presented significant tyrosinase inhibition (IC50=7.8 μg/mL). Fast centrifugal partition chromatography (FCPC) followed by various chromatographic techniques (Sephadex, semi-prep- HPLC, prep-TLC) facilitated the isolation of 15 compounds. Structure elucidation was performed with 1D & 2D NMR experiments and 8 flavonoids, 1 lignan, 1 lactone, 2 phenylpropanoids and 3 sesquiterpene lactones were identified. One flavonoid and one lactone were isolated from the genus Centaurea for the first time.


 

Publication History

Article published online:
16 November 2023

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