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DOI: 10.1055/s-0043-1774130
Comparison of In vitro Anti-inflammatory, COX-1/COX-2 and LOX inhibitory activities of Malva sylvestris L. leaves and flowers extract
Malva sylvestris L. was used traditionally for its anti-inflammatory, anti-ulcerogenic and wound-healing properties. The aim of this study was the evaluation of M. sylvestris leaf and flower extracts for their in vitro COX-1/COX-2 and LOX enzyme inhibitory activity. The extracts were separately prepared by maceration with 70% ethanol. M. sylvestris extracts (European Pharmacopoeial quality) were analysed by HPLC. MTT was used for in vitro cytotoxic effects using the HEK293/A549, MCF7 and PC3 cell lines. To evaluate the COX- 1/COX-2 inhibition, they were studied with commercial assay test kits (20 µg/mL concentration). The major component of M. sylvestris flower extract was characterised as 0.19% malvin; components of leaf extract were characterised as 0.6% tiliroside and 2.5% rutin ([Figs. 1] and [2]). The cytotoxicity result showed that there was not any cytotoxic effect on HEK293 cells and the results were statistically significant. The IC50 results for COX-1 and COX-2 inhibition of the flower extract were 62.13 and 23.77 µg/mL, for the leaf extract the results were 47.90 and 28.13 µg/mL, respectively. The inhibition value of the flower extract on LOX was 73.17% and the value of the leaf extract on LOX was 71.3%. Overall, M. sylvestris extracts showed selective potential for COX-2 enzyme inhibition. The extracts were tested against selected cancer cell lines for the first time to the best of our knowledge. Further in vivo tests are required to confirm the anti-inflammatory and anticancer potentials of the M. sylvestris extracts, also combination studies are worthwhile to screen.
Conflict of Interest
The authors declare no conflict of interest.
Publikationsverlauf
Artikel online veröffentlicht:
16. November 2023
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