Evaluation of antioxidant, anti-inflammatory and neuroprotective activities and chemical contents of species used traditionally for inflammation in Turkey

The pathophysiology of Parkinson's disease (PD) is linked to accumulation of the cytoplasmic structures called Lewy bodies with their main constituent being α-synuclein oligomers. Recent studies showed that α- synuclein overexpression is associated with microglial activation, neuroinflammation, and neurodegeneration. In order to protect neurons and prevent disease progression, studies on agents that are effective on α-synuclein expression, whose oligomers cause cell damage by initiating neuroinflammation, and therefore anti-inflammatory and antioxidant agents, are increasingly important [1].

This study aimed to determine antioxidant, anti-inflammatory, and neuroprotective activities of various extracts of Clematis vitalba, Ecballium elaterium (EE), Fraxinus ornus subsp. ornus (FOO), Paliurus spina- christi and Sambucus ebulus (SE), which might indicate a therapeutic potential for treatment of inflammatory disorders like PD. FOO showed the highest antioxidant activity by DPPH method. FOO, SE and EE extracts exhibited higher anti-inflammatory potential since they reduced nitric oxide and TNF-production by Griess assay (using LPS-activated RAW 264.7 macrophages) and TNF bioassay (using L929 cells), and were found to be rich in flavonoid and phenolic compounds chromatographically. Therefore, their protective activities on the toxicity induced by rotenone and A-53T-α-synuclein plasmid were tested by analysis of the change in α-synuclein expression, its effect on the cell lines and cell viability analysis through xCELLigence. Only FOO cortex extract was protective and decreased the cell death. Our findings indicate that FOO has good anti-inflammatory and neuroprotective activity. Further studies are required to assess its potential applications in the treatment of inflammatory disorders like PD.

 

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• References

• 1 Forloni G.. Alpha Synuclein: Neurodegeneration and lnflammation. lnt. J. Mol Sci 2023; 24: 5914

Publication History

Article published online:
16 November 2023