Evaluation of antioxidant and antidiabetic activities of Trigonella foenum-graecum phytoestrogen-rich extracts

Trigonella foenum-graecum has long been used in traditional medicine for different diseases. Recent studies highlighted the antidiabetic activity of fenugreek seeds linked to the presence of trigonelline alkaloid and galactomannan polysaccharides [1] [2]. In this study, T. foenum-graecum extract was obtained by accelerated solvent extraction (ASE), and processed by microfiltration and nanofiltration in a tangential module, using a membrane with MWCO 150-300 Da for concentrating the phytoestrogen compounds. Some of the most important phytoestrogens are flavonoids and isoflavonoids. Plant extracts were evaluated for phytochemical profile (phenolics, flavonoids, isoflavonoids), antioxidant (DPPH• and reducing power assays), α-amylase and α-glucosidase inhibition. The HPLC-MS analysis showed that the T. foenum-graecum phytoestrogen-rich extract contains mainly vitexin, rutin and isoquercitrin. The studied extract exhibited a high antioxidant capacity in DPPH inhibition (85.7±0.6%) and reducing power assay (94.7%). The T. foenum-graecum phytoestrogen-rich extract showed significant α-amylase (IC₅₀=9.71 µg/mL) and α- glucosidase (IC₅₀=28.19 µg/mL) inhibitory activities, compared to acarbose as control (IC₅₀=24.18 µg/mL and 798.32 µg/mL, respectively). Furthermore, combinations of isoflavones with other bioactive compounds from the concentrated extract could act synergistically and the T. foenum-graecum phytoestrogen-rich extract could be a feasible source of nutraceuticals.

Funding This work was supported by a grant of the Ministry of Research, Innovation and Digitization, CCCDI – UEFISCDI, project number PN-III-P2-2.1-PED-2021-1185, within PNCDI III.

 

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• References

• 1 Singh N., Yadav S.S., Kumar S., Narashiman B.. Food Biosci 2022; 46
• 2 Srinivasa U.M., Naidu M.M.. Studies in Natural Products Chemistry 2021; 71: 141-184

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Artikel online veröffentlicht:
16. November 2023