Effects of Marantodes pumilum on biochemical and histophatological changes on dyslipidemia rats model

Marantodes pumilum, also known as Labisia pumila or Kacip Fatimah by the local people, has been studied for its antioxidant, anti-inflammatory, phytoestrogenic and skin collagen synthesis promoting properties. Our previous study revealed the extracts provided potentially inhibited in vitro LDL oxidation and platelet aggregation. Our further study also revealed that the extracts had improved the in vivo antioxidant enzymes activity during isoproterenol-induced myocardial infraction events on rats. Thus, our present study aimed to evaluate the effects of M. pumilum extracts on reducing the atherosclerotic risks on high-cholesterol diet rats. The rats were induced to dyslipidemia status with a daily intake of 2% cholesterol for a duration of 8 weeks. Three different doses (100, 200 and 400 mg/kg) of the extracts were administered daily on the 4th week onwards. The rats were then sacrificed, and the blood was collected via abdominal aorta and, serum was separated by centrifugation for biochemical analysis. Part of the aorta tissues were excised immediately for histopathological examination. The results of treated rats showed significant reduction (p≤0.05) in serum aspartate aminotransferase (AST), alanine aminotransferase (ALT) and lactate dehydrogenase (LDH) levels and improved the antioxidant enzymes. The histopathological examination of the abdominal aorta showed a decrease of atheroma lesions in treated rats and significantly improved (p≤0.05) in the lipid profile which eventually reduced the atherogenic indices in treated rats. The extracts of M. pumilum can reduce the risk of atherosclerosis on dyslipidaemia rats at the initial stage by improving the serum lipid profiles and modulating serum antioxidants.

 

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Publication History

Article published online:
16 November 2023