Safety assessment of Serjania triquetra

Serjania triquetra is used in traditional medicine to treat kidney diseases and is found in various herbal products. However, with the increase in the sale of herbal products without proper validation, it is important to assess the safety and quality of herbal medicinal products to ensure the well-being of individuals using them. This can be achieved through a combination of in vitro and in vivo studies, including adherence to the OECD guidelines for toxicity testing. Safety of commercial S. triquetra products was evaluated by lethality assay in Artemia salina L. model and acute oral toxicity (AOT) in vivo (mouse) according to OECD Guide 420. Different concentrations of the hydroalcoholic extract from two commercial samples (HAES-I, HAES-II) and a reference (HAESt) were evaluated to determine its toxicity according to Meyer's and Clarkson's toxicity index. Results showed that the extracts analysed were classified as "non-toxic" with values higher than 1000 μg/mL. The AOT of HAESt was evaluated by administering doses of 300 and 1000 mg/kg intragastrically and observing behaviour and weight variation for 14 days. Signs of toxicity were observed in the animals, including changes in breathing, piloerection and pain upon touch, but no weight variations were recorded. Necropsy revealed changes in organ coloration, whitish masses in the lungs, and decreased heart size. Based on these results, HAESt was classified as Category 4 "Harmful if ingested" according to the GHS, with an LD₅₀ of>300 and≤2000 mg/kg. According to the results, S. triquetra is classified as low hazard.

 

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Artikel online veröffentlicht:
16. November 2023