Planta Med 2023; 89(14): 1400
DOI: 10.1055/s-0043-1774196
Abstracts
Wednesday 5th July 2023 | Poster Session III
Analytical Methods

Seasonal variation effects and LC-HRMS/MS monitoring of key metabolites of Pistachia lentiscus L. leaves

Theodora Nikou
1   Division of Pharmacognosy and Natural Products Chemistry, Department of Pharmacy, National and Kapodistrian University of Athens, Athens, Greece
,
Varvara Papaioannou
1   Division of Pharmacognosy and Natural Products Chemistry, Department of Pharmacy, National and Kapodistrian University of Athens, Athens, Greece
,
Aikaterini L Stefi
2   Section of Botany, Department of Biology, Faculty of Sciences, National and Kapodistrian University of Athens, Athens, Greece
,
Dido Vassilakopoulou
3   Section of Biochemistry and Molecular Biology, Department of Biology, Faculty of Sciences, National and Kapodistrian University of Athens, Athens, Greece
,
Nikolaos S Christodoulakis
2   Section of Botany, Department of Biology, Faculty of Sciences, National and Kapodistrian University of Athens, Athens, Greece
,
Maria Halabalaki
1   Division of Pharmacognosy and Natural Products Chemistry, Department of Pharmacy, National and Kapodistrian University of Athens, Athens, Greece
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Mastic tree (Pistacia lentiscus – Anacardiaceae), is a dioecious evergreen sclerophyllous shrub native to the Mediterranean Basin, mostly cultivated for its aromatic resin. The leaves of the shrub are considered as a valuable source of phenolic compounds, as plants of the Mediterranean-type climate, and produce increased amounts of secondary metabolites [1]. However, leaves are an underestimated part of P. lentiscus, and limited literature data exist for their phytochemical profile. Thus, the aim of the current study was the phytochemical investigation of P. lentiscus leaves and the correlation of their metabolic profile to seasonal variations and thus temperature conditions. Seeds of Pistacia lentiscus were cultured in plant growth chambers for three months and separated into three groups. The first group was left to grow under normal Mediterranean conditions, as those recorded in spring. The other group was exposed to a five-day heat stress while the last one suffered a cold stress for five days as well. Leaves were successively extracted (DCM, MeOH, MeOH/H2O 1/1) and then analysed via UPLC-qTOF MS for compound identification. Interesting correlations were revealed, indicating that summer and winter leaves (stressed plants) displayed similar qualitative profile, although with evident quantitative differences. Additionally, specialised protocols were developed for the quantitation of key-biosynthetic metabolites, verifying the observed qualitative variations, while indicating that plant exposure to specific temperatures shifts the secondary metabolic pathways and influences the biosynthesis of certain metabolites.

Funding ERDF, “RESEARCH–CREATE- INNOVATE”, Hyper-Mastic (project code Τ2ΕΔΚ-00547)


 

Conflict of Interest

The authors declare no conflict of interest.


Publication History

Article published online:
16 November 2023

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