Planta Med 2023; 89(14): 1405-1406
DOI: 10.1055/s-0043-1774211
Abstracts
Wednesday 5th July 2023 | Poster Session III
Molecular modelling/ Virtual screening/ Metabolomics/Molecular networking/ Chemometrics and profiling

Untargeted metabolomics based on UPLC-MS coupled with a chemometrics approach to target α-glucosidase inhibitory activity of six Panamanian Cecropia species

Aylin De Lora
1   Departamento de Química Medicinal y Farmacognosia, Facultad de Farmacia, Universidad de Panamá, Ciudad de Panamá, Panamá
,
Yuliana Christopher
1   Departamento de Química Medicinal y Farmacognosia, Facultad de Farmacia, Universidad de Panamá, Ciudad de Panamá, Panamá
,
Orlando Ortiz
4   University of Munich, Germany
,
Yostin Añino
5   Museo de Invertebrados G. B. Fairchild, Universidad de Panamá, Ciudad de Panamá, Panamá
,
Yancho Zarev
6   Department of Pharmacognosy, Faculty of Pharmacy, Medical University of Sofia, 2 Dunav Str., 1000, Bulgaria
,
Andrés Rivera- Mondragón
1   Departamento de Química Medicinal y Farmacognosia, Facultad de Farmacia, Universidad de Panamá, Ciudad de Panamá, Panamá
2   Instituto Especializado de Análisis (IEA), Universidad de Panamá, Ciudad de Panamá, Panamá
3   Sistema Nacional de Investigación, Senacyt, Ciudad de Panamá, Panamá
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    Guarumo leaf (Cecropia sp.) is a Latin American medicinal plant that has been traditionally used to treat diabetes mellitus. Due to the lack of comprehensive research, its antidiabetic mechanism has not yet been fully described. One of the therapeutic approaches in the management of type 2 diabetes is preventing a rise in postprandial glucose levels through the inhibition of intestinal α-glucosidase. In consequence, this work aimed to evaluate the potential α-glucosidase inhibitory activity of the leaf extracts of six Cecropia species collected in Panama and to characterise the chemical compounds related to such activity. Since the classical blinded-chemical investigation is a challenging process due to the highly complex chemical composition of medicinal plants, we used untargeted metabolomics-based ultra-high-performance liquid- chromatography–tandem mass spectrophotometry (UPLC–MS/MS) coupled with an orthogonal projection to latent structure (OPLS) for screening the most probable bioactive candidates. Out of all Cecropia species evaluated, C. obtusifolia, C. hispidissima, C. longipes, C. peltata (Cerro Azul), and C. insignis showed significant α-glucosidase inhibitory activity with IC50 values in the range of 9.0 – 44.0 mg/mL, compared to acarbose (4977.1 mg/mL) as a positive control. C. heterochroma and C. peltata (collected in Sta. Catalina) did not show any significant effect in this assay. Procyanidin B, ourateacatechin (4′-Methyl- epigallocatechin) and kaji-ichigoside F1 were correlated to be the strongest inhibitors of α-glucosidase via the OPLS model. Our results suggest that these Cecropia constituents are promising candidates for the development of an anti-hyperglycemic formulation to manage postprandial hyperglycemia incidence ([Fig. 1]).

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    Fig. 1 Metabolomics results.

     

    Publication History

    Article published online:
    16 November 2023

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    Fig. 1 Metabolomics results.