Planta Med 2023; 89(14): 1407
DOI: 10.1055/s-0043-1774216
Abstracts
Wednesday 5th July 2023 | Poster Session III
Molecular modelling/ Virtual screening/ Metabolomics/Molecular networking/ Chemometrics and profiling

HPLC Analysis on Ginseng Root in Normal and Root Rot model

Minsik Choi
1   Department of Biomedical and Pharmaceutical Sciences, Graduate School, Kyung Hee University, Seoul, South Korea
,
Geonha Park
2   Department of Life and Nanopharmaceutical Sciences, Graduate School, Kyung Hee University, Seoul, South Korea
,
Sejin Ku
1   Department of Biomedical and Pharmaceutical Sciences, Graduate School, Kyung Hee University, Seoul, South Korea
,
Kyu Won Kim
1   Department of Biomedical and Pharmaceutical Sciences, Graduate School, Kyung Hee University, Seoul, South Korea
,
Young Pyo Jang
1   Department of Biomedical and Pharmaceutical Sciences, Graduate School, Kyung Hee University, Seoul, South Korea
2   Department of Life and Nanopharmaceutical Sciences, Graduate School, Kyung Hee University, Seoul, South Korea
3   Department of Integrated Drug Development and Natural Products, College of Pharmacy, Kyung Hee University, Seoul, South Korea
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    Although ginseng is highly valued worldwide for its pharmacological benefits, its mass production is challenging due to its uneconomical production process. The growth of ginseng is heavily influenced by soil microbes and the environment, making it vulnerable to diseases such as root rot, which is a major obstacle to ginseng production. While methods to cope with this disease are being researched, there is currently a lack of metabolic research related to ginseng root rot. To address this issue, an experiment was designed to identify the molecular components of ginseng that are related to root rot disease by comparing normal and diseased ginseng extracts using HPLC-PDA. From multivariate analysis on the HPLC chromatograms, ten significant peaks were identified, and six substances with VIP scores over 1.0 were selected for further analysis: ginsenoside Rg1, ginsenoside Re, ginsenoside Ra1, ginsenoside Rc, notoginsenoside Fc, and panaxydol. By taking a metabolomics approach to understand ginseng root rot related metabolome change, it was discovered that these six substances are primarily responsible for the disease in continuously cropped ginseng. This finding will lay the foundation for future metabolomics research on ways to find efficient cultivation methods for the continuous cropping of ginseng.


     

    Conflict of Interest

    The authors declare no conflict of interest.

    Publication History

    Article published online:
    16 November 2023

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