The effect of Pouteria campechiana fruit extracts on the inhibition of cancer cell proliferation

This study aimed to test the effect of Pouteria campechiana fruit extracts on inhibiting the growth of cancer cells and to investigate the molecular mechanism of Pouteria campechiana fruit extract-induced apoptosis. The MTT assay was used to assess the cell viability of HT-29 cells inhibited by Pouteria campechiana fruit extracts compared with doxorubicin (positive control) and without Pouteria campechiana fruit extracts (negative control) and percentage of cell viability of FHC treated by Pouteria campechiana fruit extracts compared with doxorubicin (positive control) and without Pouteria campechiana fruit extracts (negative control). The MTT assay was determined using a UV spectrophotometer technique then converted into percentage of cell viability. Moreover, morphological change of HT-29 and FHC cells was observed by Phase contrast microscopy and the mechanism of Pouteria campechiana fruit extracts in inhibiting the growth of cancer cells was determined by Western blot. The MTT assay revealed that Pouteria campechiana fruit extracts significantly inhibited the cell viability of HT-29 cells in a concentration and time dependent manner. The cell viability of FHC normal cells treated with Pouteria campechiana fruit extracts at 24, 48 and 72 hours were more than 90% and not decreased with higher concentration. Phase contrast microscopy of HT-29 cells showed the morphology changes of cells treated with extract included shrinkage and fragmentation. Western blot analysis showed the change of apoptotic related proteins including caspase-3, PARP-1, survivin and Bid. This study indicated that Pouteria campechiana fruit extract inhibited HT-29 cell proliferation by apoptosis through apoptosis inducing proteins.

 

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Artikel online veröffentlicht:
16. November 2023