Planta Med 2023; 89(14): 1428
DOI: 10.1055/s-0043-1774280
Abstracts
Wednesday 5th July 2023 | Poster Session III
Phytopharmacology III – Pharmaceutics

Poly(3-hydroxybutyrate)-based composite 3D printed scaffolds for tissue engineering applications

Vasileios Manouras
1   Aristotle University of Thessaloniki, School of Chemical Engineering, Laboratory of Organic Chemistry, Thessaloniki, Greece
,
Konstantinos Theodoridis
1   Aristotle University of Thessaloniki, School of Chemical Engineering, Laboratory of Organic Chemistry, Thessaloniki, Greece
,
Athanasios Arampatzis
1   Aristotle University of Thessaloniki, School of Chemical Engineering, Laboratory of Organic Chemistry, Thessaloniki, Greece
2   Center for Interdisciplinary Research and Innovation of Aristotle University of Thessaloniki, Natural Products Research Center of Excellence (NatPro-AUTH), Thessaloniki, Greece
,
Evangelos Kyrilas
3   Aristotle University of Thessaloniki, Faculty of Engineering, School of Chemical Engineering & Physics Laboratory, Thessaloniki, Greece
,
Elli Kampasakali
3   Aristotle University of Thessaloniki, Faculty of Engineering, School of Chemical Engineering & Physics Laboratory, Thessaloniki, Greece
,
Ioannis Tsivintzelis
4   Aristotle University of Thessaloniki, School of Chemical Engineering, Laboratory of Physical Chemistry, Thessaloniki, Greece
,
Lazaros Tsalikis
5   Aristotle University of Thessaloniki, School of Dentistry, Thessaloniki, Greece
,
Christina Papanikolaou
6   Aristotle University of Thessaloniki, School of Chemical Engineering, Laboratory of Biochemical and Biotechnological Processes, Thessaloniki, Greece
,
Christos Chatzidoukas
6   Aristotle University of Thessaloniki, School of Chemical Engineering, Laboratory of Biochemical and Biotechnological Processes, Thessaloniki, Greece
,
Panagiotis Barmpalexis
2   Center for Interdisciplinary Research and Innovation of Aristotle University of Thessaloniki, Natural Products Research Center of Excellence (NatPro-AUTH), Thessaloniki, Greece
7   Aristotle University of Thessaloniki, School of Pharmacy, Laboratory of Pharmaceutical Technology, Thessaloniki, Greece
,
Dimitrios Christofilos
3   Aristotle University of Thessaloniki, Faculty of Engineering, School of Chemical Engineering & Physics Laboratory, Thessaloniki, Greece
,
Andreana Assimopoulou
1   Aristotle University of Thessaloniki, School of Chemical Engineering, Laboratory of Organic Chemistry, Thessaloniki, Greece
2   Center for Interdisciplinary Research and Innovation of Aristotle University of Thessaloniki, Natural Products Research Center of Excellence (NatPro-AUTH), Thessaloniki, Greece
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    Poly(hydroxybutyrate) or PHB is a natural bio-based aliphatic polyester, which is degradable by numerous microorganisms (bacteria, fungi and algae) [1]. PHB can be produced from bacterial fermentation under nutrient-limiting conditions. Poly(3-hydroxy-butyrate) is considered a non-toxic, highly biodegradable and biocompatible material making it very attractive for various biomedical applications [2]. It is a versatile natural polymer that can be extruded, moulded, spun into fibres or processed into films. However, high production costs, thermal instability, and disputable mechanical properties limit its desirable applications. Copolymers or blends with other polymers can broaden its clinical applications, with enhanced characteristics on demand [3]. PHB alone or with various combinations, has been recently used to create porous structures with a sol-gel approach, suitable for bone tissue engineering scaffolds [4].

    In order to improve printability of the PHB material, we have synthesised several blends of PHB with other biocompatible polyesters, namely poly(ε-caprolactone) (PCL) and poly(lactic acid) (PLA). Each polymer was diluted in chloroform and their solutions were mixed in appropriate ratios. The composite materials were successfully fabricated as 3D-printed bioscaffolds with the BIO-X 3D-bioprinter (CELLINK​, Sweden) using a thermoplastic head. Morphological characteristics of these scaffolds were examined by Scanning Electron Microscopy (SEM), and their physicochemical properties were assessed with Differential Scanning Calorimetry (DSC), Thermogravimetric Analysis (TGA), Fourier Transform Infrared Spectroscopy (FT-IR) and Raman spectroscopy. This work focuses on the exploitation of composite materials based on the natural- based PHB to fabricate 3D printed bioscaffolds for replacing damaged bone tissues or tissues that cannot self-regenerate, such as cartilage tissue.


     

    Publication History

    Article published online:
    16 November 2023

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