Abstract
Samples of Hyperici herba were obtained by harvesting Hypericum perforatum L. in different plant development stages. The relation of flower development phases
in the drug's flower fraction was examined. The HPLC method was then employed for
the analysis of the content of secondary metabolites in different flower ontogenesis
phases. The content of dianthrones, derivatives of quercetin and hyperforin increased
from the first bud phases (0.29 %, 0.80 %, and 2.47 %, respectively) to flowers just
opened (1.04 %, 4.23 % and 6.60 %, respectively). The content of dianthrones and quercetin
glycosides then decreased (in unripe fruits 0.11 % and 0.08 %, respectively), whereas
the amounts of hyperforin increased to 8.07 % in fruits. The content of I3,II8-biapigenin
increased from 0.21 % in small buds to 1.04 % in buds just before opening and has
then decreased gradually to a value of 0.02 % in fruits. Rutin was not detected in
the samples.
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Dr. Daniela Tekel'ová
Department of Pharmacognosy and Botany Faculty of Pharmacy, Comenius University
Odbojárov 10
SK-83232 Bratislava
Slovak Republic
Email: tekelova@fpharm.uniba.sk
Phone: +421 7 55572065