Abstract
Tissue damage as a result of oxygen radical generation may be involved in the pathogenesis
of different diseases, carcinogenesis, aging and cell death. The inhibition of the
proliferation rate of the immortalised human cell line ECV 304 after oxidant damage
by oxygen radicals generated in a hypoxanthine-xanthine oxidase system and the protection
provided by some selected flavone and flavonol glycosides as well as by caffeic acid
and its derivatives was determined. The cytotoxicity of the reactive oxygen species
was differentially influenced by selected flavonoids and seems to be determined by
the pattern of substitution and by their lipophilicity. Apigenin and quercetin demonstrated
the strongest effect on the inhibition of hypoxanthine-xanthine oxidase-induced toxicity
(50 % restitution of the cells at a concentration of 0.36 μM and 3.1 μM, respectively).
The beneficial effect of the flavonol glycosides rutin and hyperoside was weak, whereas
flavone glycosides such as diosmin showed a better effect of protection.
Key words
ECV 304-cells - Hypoxanthine - xanthine oxidase - radical scavenger activity - flavonols
- flavones - caffeic acid derivatives
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Prof. M. F. Melzig
Institut für Pharmazie
Freie Universität Berlin
Königin-Luise-Str. 2
14195 Berlin
Germany
Phone: +49-30-838-51451
Fax: +49-30-838-51461
Email: melzig@zedat.fu-berlin.de
