Abstract
Sequences of 5S rRNA gene spacer were used to identify Epimedium brevicornu Maxim., E. sagittatum (Sieb. et Zucc.) Maxim., E. wushanense T. S. Ying, E. pubescens Maxim., and E. koreanum Nakai. These species are listed as source plants of Chinese medicine ‘Ying Yang Huo’
in the Chinese Pharmacopoeia. The neighbor-joining method was used in a sequence analysis
of Epimedium species. A position-specific nucleotide was found in the 5S rRNA gene spacer for
E. pubescens, E. wushanense, and E. brevicornu. A 19-bp deletion was found for E. koreanum in the 5S rRNA gene spacer. E. koreanum was most divergent from the other four endemic Chinese species of Epimedium.
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Prof. Pang-Chui Shaw
Department of Biochemistry
The Chinese University of Hong Kong
Shatin, N.T.
Hong Kong
China
Fax: +852-26035123
eMail: pcshaw@cuhk.edu.hk
