Planta Med 2005; 71(8): 717-720
DOI: 10.1055/s-2005-871252
Original Paper
Pharmacology
© Georg Thieme Verlag KG Stuttgart · New York

Protective Effect of the 5-Lipoxygenase Inhibitor Ardisiaquinone A on Hepatic Ischemia-Reperfusion Injury in Rats

Nobuaki Matsui1 , Nobuyuki Fukuishi1 , Yoshiyasu Fukuyama1 , Yumiko Yasui1 , Masaaki Akagi1
  • 1Faculty of Pharmaceutical Sciences, Tokushima Bunri University, Tokushima City, Japan
Further Information

Publication History

Received: October 13, 2004

Accepted: April 4, 2005

Publication Date:
29 July 2005 (online)

Abstract

The protective effects of the 5-lipoxygenase inhibitor ardisiaquinone A, a compound isolated from Ardisia sieboldii, on hepatic ischemia-reperfusion (I/R) injury was studied in rats. Hepatic I/R injury was induced by occlusion of the portal vein and the hepatic artery for 60 min, followed by reperfusion for 24 h. The content of leukotriene B4 (LTB4) in the liver increased during ischemia. Serum alanine aminotransferase (ALT) levels, a marker of hepatic parenchymal cell injury, and hepatic myeloperoxidase (MPO) activity, a marker of neutrophil accumulation, significantly increased 6 - 9 h after reperfusion. Treatment with ardisiaquinone A (0.1 - 2 mg/kg, i. p.) 30 min prior to ischemia dose-dependently prevented the increase in LTB4 content during ischemia (ID50 = 0.645 mg/kg) with a slightly higher potency than that of AA-861 (ID50 = 0.728 mg/kg), a known reference 5-lipoxygenase inhibitor. Ardisiaquinone A also attenuated the increase in MPO activity and serum ALT levels at 6 h after reperfusion (ID50 = 1.71 mg/kg and 4.28 mg/kg, respectively). These protective effects were more efficient than those of AA-861 (ID50 = 1.86 mg/kg and no effect, respectively), LY255283 (ID50 = 18.1 mg/kg and 11.5 mg/kg, respectively), and ONO-4057 (ID50 = 8.38 mg/kg and 9.44 mg/kg, respectively), which are LTB4 receptor antagonists. These results suggest that ardisiaquinone A may protect the liver against damage due to I/R in rats.

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Nobuaki Matsui

Department of Pharmacology

Faculty of Pharmaceutical Sciences

Tokushima Bunri University

180 Nishihama-bouji

Yamashiro-cho

Tokushima City

Tokushima 770-8514

Japan

Phone: +81-88-622-9611 ext. 5722

Fax: +81-88-655-3051

Email: matsui@ph.bunri-u.ac.jp

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