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Planta Med 2005; 71(10): 933-937
DOI: 10.1055/s-2005-871283
Original Paper
Pharmacology
© Georg Thieme Verlag KG Stuttgart · New York

Effect of Three Flavonoids Isolated from Japanese Polygonum Species on Superoxide Generation in Human Neutrophils

Gang Liu1 , 4 , Weifang Wang1 , Noriyoshi Masuoka2 , Takahiko Isobe3 , Koichi Yamashita1 , Masanobu Manabe1 , Hiroyuki Kodama1
  • 1Department of Anesthesiology and Critical Care Medicine, Kochi Medical School, Kochi, Japan
  • 2Department of Biochemistry, Okayama University Graduate School of Medicine and Dentistry, Okayama, Japan
  • 3Department of Chemistry, Hyogo College of Medicine, Hyogo, Japan
  • 4Department of Anesthesiology, First Affiliated Hospital of China Medical University, Shenyang, P. R. China
Weitere Informationen

Publikationsverlauf

Received: December 21, 2004

Accepted: April 13, 2005

Publikationsdatum:
19. August 2005 (online)

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Abstract

Three flavonoids, quercetin 3-O-glucoside-2′′-gallate (QGG), quercetin 3-O-rhamnoside-2′′-gallate (QRG) and kaempferol 3-O-glucoside-2′′-gallate (KGG) were isolated from Japanese Polygonum species. The effect of these flavonoids on stimulus-induced superoxide generation in human neutrophils was assayed by measuring the reduction of cytochrome c. The tyrosyl or serine/threonine phosphorylation of neutrophil proteins and the translocation of p47phox and p67phox to the cell membrane were detected using specific monoclonal antibodies. The flavonoids used in this experiment significantly suppressed stimulus-induced superoxide generation in a concentration-dependent manner. FMLP-induced tyrosyl phosphorylation or PMA-induced serine/threonine phosphorylation and the translocation of the cytosolic proteins p47phox and p67phox to the cell membrane were suppressed in parallel to the suppression of the stimulus-induced superoxide generation.

Key words

Polygonum - flavonoids - superoxide - human neutrophils - cytosolic compounds

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Prof. Hiroyuki Kodama

Department of Anesthesiology and Critical Care Medicine

Kochi Medical School

Nankoku-shi

Kochi 783-8505

Japan

Fax: +81-88-865-5937

eMail: hkodama@kochi-ms.ac.jp

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