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DOI: 10.1055/s-2005-871296
© Georg Thieme Verlag KG Stuttgart · New York
Endothelial Nitric Oxide Production Stimulated by the Bioflavonoid Chrysin in Rat Isolated Aorta
Publication History
Received: November 11, 2004
Accepted: April 25, 2005
Publication Date:
19 August 2005 (online)
Abstract
In the present study, the effects of the bioflavonoid chrysin (5,7-dihydroxyflavone) were analysed on nitric oxide (NO) production from vascular endothelium. In aortic rings, incubation with chrysin or acetylcholine (both at 10 μM) increased L-NAME-sensitive endothelial NO release as measured using the fluorescent probe 4,5-diaminofluorescein diacetate (DAF-2 DA). Moreover, chrysin increased cGMP accumulation only in aortic rings with endothelium. However, at this concentration, chrysin had no effect either on basal or on NADPH-stimulated vascular superoxide production. Moreover, at this low concentration, chrysin, similar to acetylcholine, induced aortic relaxation, which was abolished by both endothelial deprivation and NO synthase inhibition. Endothelium-dependent relaxation induced by chrysin was unaltered by removal of extracellular calcium and incubation with the intracellular calcium chelator BAPTA, while the phosphatidylinositol (PI)-3 kinase inhibitor wortmannin suppressed the endothelial dependence. In conclusion, chrysin stimulated NO release from endothelial cells leading to vascular cGMP accumulation and subsequent endothelium dependent aortic relaxation. Chrysin-stimulated NO release is calcium independent and possibly mediated via PI3-kinase.
Key words
Chrysin - flavonoids - thoracic aorta - NO - cGMP - Superoxide
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J. Duarte
Departamento de Farmacología
Facultad de Farmacia
Universidad de Granada
18071 Granada
Spain
Phone: +34-958-244-088
Fax: +34-958-248-964
Email: jmduarte@platon.ugr.es