Authentication of Medicinal Plant Botanical Identity by Amplified Fragmented Length Polymorphism Dominant DNA Marker: Inferences from the Plectranthus Genus

Helna Passinho-Soares; Durvalina Felix; Maria Auxiliadora Kaplan; Marcia Margis-Pinheiro; Rogério Margis

doi:10.1055/s-2006-946673

Planta Med 2006; 72(10): 929-931
DOI: 10.1055/s-2006-946673

Letter

Authentication of Medicinal Plant Botanical Identity by Amplified Fragmented Length Polymorphism Dominant DNA Marker: Inferences from the Plectranthus Genus

Helna Passinho-Soares¹ ^, ² , Durvalina Felix¹ , Maria Auxiliadora Kaplan³ , Marcia Margis-Pinheiro⁵ , Rogério Margis¹ ^, ⁴

¹Departamento de Genética, UFRJ, Rio de Janeiro, RS, Brasil
²Departamento do Medicamento, Faculdade de Farmácia, UFBa, Salvador, BA, Brasil
³Núcleo de Pesquisas de Produtos Naturais, UFRJ, Rio de Janeiro, Brasil
⁴Universidade Federal do Rio Grande do Sul, Porto Alegre, RS, Brasil
⁵Departamento de Genética, UFRGS, Porto Alegre, RS, Brasil

Abstract

In Brazil, Plectranthus species are known as ”boldo” and have been used in popular medicine for analgesic and dyspeptic purposes. Plectranthus need to be well identified in order to be used as commercially genuine medicinal plants. Here we describe AFLP DNA patterns able to distinguish among different Pectranthus species. The genetic variability of P. grandis Cramer, P. barbatus Andr. and P. ornatus Codd was analyzed with two sets of AFLP primers allowing detection of 241 loci. A total of 22 monomorphic loci were identified in P. barbatus, 15 in P. grandis and 30 in P. ornatus. Among these, 5 loci were informative and species-specific to P. barbatus, 3 to P. grandis and 2 loci were unique to P. ornatus. The AFLP pattern analyzed by different clustering methods assembled individuals according to their species. So far, AFLP represents a genuine and strong method to certify medicinal plant materials.

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Dr. Rogério Margis

Universidade Federal do Rio Grande do Sul

Rua Ramiro Barcelos

2600 - Prédio anexo

Bairro Santana

Porto Alegre RS

Brasil CEP 90.035-003

Phone: +55-51-3316-7766

Email: rogerio.margis@ufrgs.br

Supplementary Material

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