Anti-Asthmatic Effects of Perilla Seed Oil in the Guinea Pig in vitro and in vivo

Abstract

The aim of this study was to investigate the anti-asthmatic effects of Perilla seed oil in vitro and in vivo in sensitized guinea pigs. Aerosolized antigen caused an immediate bronchoconstriction. Perilla seed oil per os inhibited the increase in lung resistance and the decrease in dynamic lung compliance in a dose-dependent manner with an ED₅₀ (95 % confidence interval, CI) of 1.10 (0.98 - 1.24) g/kg and 1.07 (0.94 - 1.22) g/kg, respectively. Infiltration of leukocytes, mononuclear cells, eosinophils and neutrophils induced by inhaling antigen was also inhibited by Perilla seed oil in a dose-dependent manner with an ED₅₀ (95 % CI) of 1.00 (0.86 - 1.15), 1.24 (1.10 - 1.38), 0.63 (0.51 - 0.77) and 0.61 (0.38 - 0.98) g/kg, respectively. Perilla seed oil (5 - 500 μg/mL) inhibited the slow reaction substance of anaphylaxis (SRS-A) release induced by antigen challenge in lung tissue of sensitized guinea pigs. It also inhibited calcium ionophore (A₂₃₁₈₇)-induced leukotriene (LT) D₄ release from the lung tissue of non-sensitized guinea pigs in a concentration-dependent manner with an IC₅₀ (95 % CI) of 50 (36 - 69) μg/mL. These results indicate that Perilla seed oil may improve lung function in asthma by controlling eicosanoid production and suppressing LT generation.

Key words

Asthma - Perilla - Perilla frutescens (L.) Britton - Labiatae - Inflammation - leukotrienes

References

• 1 Longvah T, Deosthale Y G. Chemical and nutritional studies on Hanshi (Perilla frutescens) a traditional oil seed from Northeast India.  J Am Oil Chem Soc. 1991;  68 781-4
• 2 Schwartz J. Role of polyunsaturated fatty acids in lung disease.  Am J Clin Nutr. 2000;  71 393S-6S
• 3 Prescotta S L, Calder P C. N-3 polyunsaturated fatty acids and allergic disease.  Curr Opin Clin Nutr Metab Care. 2004;  7 123-9
• 4 Schachter H M, Reisman J, Tran K, Dales B, Kourad K, Barnes D. et al .Health effects of omega-3 fatty acids on asthma. Agency for Healthcare Research and Quality (AHRQ). Publication 2004 No. 4-E013 - 2
• 5 Zhang Y P, Zhang H, Wang W H, Zhang S H. Refinement of Perilla seed oil and purification of α-linolenic acid from oil.  J Chin Cereals Oils Assoc. 1999;  14 41-4
• 6 Xie Q M, Chen J Q, Shen W H, Yang Q H, Bian R L. Comparison of bronchodilating and antiinflammatory activities of oral formoterol and its (R,R)-enantiomers.  Acta Pharmacol Sin. 2003;  24 277-82
• 7 Fleisch J H, Haisch K D, Spaethe S M. Slow reacting substance of anaphylaxis (SRS-A) release from guinea-pig lung parenchyma during antigen- or ionophore-induced contraction.  J Pharmacol Exp Ther. 1982;  221 146-51
• 8 Deng Y M, Xie Q M, Chen J Q, Bian R L. Coincidental increase of leukotriene B4 between cerebral cortex and lung tissue of sensitized rats.  Acta Pharmacol Sin. 2003;  24 1039-44
• 9 Powell W S. Reversed-phase high-pressure liquid chromatography of arachidonic acid metabolites formed by cyclooxygenase and lipoxygenases.  Anal Biochem. 1985;  148 59-69
• 10 National Institutes of Health NHLBI. Burden of asthma. Available at http://www.ginasthma.com/workshop.pdf. Accessed July 28, 2004
• 11 Hemelaers L, Henket M, Sele J, Bureau F, Louis R. Cysteinyl-leukotrienes contribute to sputum eosinophil chemotactic activity in asthmatics.  Allergy. 2006;  61 136-9
• 12 Simopoulos A P. Omega-3 fatty acids in inflammation and autoimmune diseases.  J Am Coll Nutr. 2002;  21 495-505
• 13 Okamoto M, Mitsunobu F, Ashida K, Mifune T, Hosaki Y, Tsugeno H. et al . Effects of Perilla seed oil supplementation on leukotriene generation by leucocytes in patients with asthma associated with lipometabolism.  Int Arch Allergy Immunol. 2000;  122 137-42
• 14 Rodriguez-Cruz M, Tovar A R, del Prado M, Torres N. Molecular mechanisms of action and health benefits of polyunsaturated fatty acids.  Rev Invest Clin. 2005;  57 457-72
• 15 Okamoto M, Mitsunobu F, Ashida K, Misune T, Hosaki Y, Tsugeno H. et al . Effects of dietary supplementation with n-3 fatty acids compared with n-6 fatty acids on bronchial asthma.  Intern Med. 2000;  39 107-11
• 16 Shin T Y, Kim S H, Kim S H, Kim Y K, Park H J, Chae B S. et al . Inhibitory effect of mast cell-mediated immediate-type allergic reactions in rats by Perilla frutescens.  Immunopharmacol Immunotoxicol. 2000;  22 489-500
• 17 Gu J Y, Wakizono Y, Dohi A, Nonaka M, Sugano M, Yamada K. Effect of dietary fats and sesamin on the lipid metabolism and immune function of Sprague-Dawley rats.  Biosci Biotechnol Biochem. 1998;  62 1917-24
• 18 Hashimoto A, Katagiri M, Torii S, Dainake J, Ichikawa A, Okuyama H. Effect of dietary α-linolenate/linoleate balance on leukotriene production and histanmine release in rats.  Prostaglandins. 1988;  36 3-16
• 19 Imaoka K, Inouye S, Takahashi T, Kojima Y. Effects of Perilla frutescens extract on anti-DNP IgE antibody production in mice.  Arerugi. 1993;  42 74-80
• 20 James M J, Gibson R A, Cleland L G. Dietary polyunsaturated fatty acids and inflammatory mediator production.  Am J Clin Nutr. 2000;  71 S343-8