Planta Med 2008; 74(11): 1380-1383
DOI: 10.1055/s-2008-1081300
Pharmacology
Letter
© Georg Thieme Verlag KG Stuttgart · New York

1,2,3,4,6-Penta-O-galloyl-β-D-glucose from Galla Rhois Protects Primary Rat Hepatocytes from Necrosis and Apoptosis

Eun-Jeon Park1 , Yu-Zhe Zhao1 , Ren-Bo An2 , 3 , Youn-Chul Kim2 , Dong Hwan Sohn1
  • 1Institute of Pharmaceutical Research and Development, Department of Pharmacy, Wonkwang University, Iksan, Republic of Korea
  • 2Laboratory of Pharmacognosy, Department of Pharmacy, Wonkwang University, Iksan, Republic of Korea
  • 3College of Pharmacy, Yanbian University, Yanji, Jilin, P. R. China
Further Information

Publication History

Received: February 28, 2008 Revised: May 25, 2008

Accepted: May 28, 2008

Publication Date:
11 July 2008 (online)

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Abstract

In this study, we investigated the hepatoprotective effects of four compounds from Galla Rhois [gallic acid methyl ester, gallic acid, an equilibrium mixture of 3-galloyl-gallic acid and 4-galloyl-gallic acid isomers, and 1,2,3,4,6-penta-O-galloyl-β-D-glucose (PGG)] in primary rat hepatocytes undergoing necrosis or apoptosis. Treatment with gallic acid methyl ester (12.5 and 50 μM) or PGG (3.125, 12.5 and 50 μM) reduced hepatocyte necrosis induced by tert-butyl hydroperoxide. PGG treatment (4 and 20 μM) also altered hepatocyte apoptosis induced by glycochenodeoxycholic acid. Based on these results, we propose that PGG warrants further evaluation as a hepatoprotective agent, because it protected primary rat hepatocytes from both necrosis and apoptosis.

Abbreviations

GA:gallic acid

GAME:gallic acid methyl ester

GCDC:glycochenodeoxycholic acid

GGA:equilibrium mixture of 3-galloyl-gallic and 4-galloyl-gallic acids

JNK:c-jun-NH2-terminal kinase

LDH:lactate dehydrogenase

PARP:poly(ADP-ribose) polymerase

PGG:1,2,3,4,6-penta-O-galloyl-β-D-glucose

tBH:tert-butyl hydroperoxide

References

Prof. Dong Hwan Sohn

Department of Pharmacy

Wonkwang University

Iksan

Jeonbuk 570–749

Republic of Korea

Phone: +82-63-850-6822

Fax: +82-63-854-6038

Email: dhsohn@wonkwang.ac.kr