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CC BY-NC-ND 4.0 · Planta Med 2022; 88(03/04): 274-281
DOI: 10.1055/a-1726-9291
Biological and Pharmacological Activity
Original Papers

Screening of Antimicrobial Activity of Essential Oils against Bovine Respiratory Pathogens – Focusing on Pasteurella multocida

Doris Bismarck
1   Laboklin GmbH & Co.KG, Bad Kissingen, Germany
,
Jens Becker
2   Clinic for Ruminants, Vetsuisse-Faculty, University of Bern, Bern, Switzerland
,
Elisabeth Müller
1   Laboklin GmbH & Co.KG, Bad Kissingen, Germany
,
Vera Becher
3   SaluVet GmbH, Bad Waldsee, Germany
,
Lisa Nau
3   SaluVet GmbH, Bad Waldsee, Germany
,
Philipp Mayer
3   SaluVet GmbH, Bad Waldsee, Germany
› Author AffiliationsSupported by: SaluVet GmbH
Supported by: Laboklin GmbH & Co.KG
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Abstract

Administration of essential oils as natural plant products with antimicrobial activity might be an alternative to antibiotic treatment of bovine respiratory disease. The aim of this study was to analyse the in vitro antimicrobial activity of 11 essential oils against Pasteurella multocida isolated from the respiratory tract of calves using microdilution with determination of minimum inhibitory and bactericidal concentration as well as agar disc diffusion. Additionally, antimicrobial activity against Mannheimia haemolytica and bacteria in the Mannheimia clade was assessed by agar disc diffusion. Seven essential oil mixtures were also tested against all bacterial isolates. P. multocida was strongly inhibited by cinnamon cassia and lemongrass oil followed by coriander, winter savory, thyme, clove, and peppermint oil in the microdilution assays. Eucalyptus, wintergreen, spruce, and star anise oil showed lower activity. Comparison of both methods revealed an underestimation of cinnamon cassia oil activity by agar disc diffusion and conflicting results for wintergreen oil in microdilution, which precipitated in broth. Cinnamon cassia, thyme, wintergreen, lemongrass, and winter savory oil all showed strong antimicrobial activity against M. haemolytica. Bacteria in the Mannheimia clade were mostly inhibited by cinnamon cassia and thyme oil. Pasteurella isolates were more susceptible to inhibition by essential oils than Mannheimia isolates. Essential oil mixtures did not show stronger antibacterial activity than single essential oils. In conclusion, cinnamon cassia and lemongrass as well as coriander, winter savory, and thyme oil are promising candidates for treatment of P. multocida-associated bovine respiratory infections.

Key words

essential oils - Pasteurella multocida - Mannheimia haemolytica - bovine respiratory disease

Supporting Information

    Supporting Information includes graphics on the EO mixture activity against P. multocida, M. haemolytica, and Mannheimia clade (Fig. 1S) and on the activity of EO mixtures and their single EO constituents against P. multocida, M. haemolytica, and Mannheimia clade (Fig. 2S).

  • Supporting Information


Publication History

Received: 28 February 2021

Accepted after revision: 13 December 2021

Article published online:
18 February 2022

© 2021. The Author(s). This is an open access article published by Thieme under the terms of the Creative Commons Attribution-NonDerivative-NonCommercial License, permitting copying and reproduction so long as the original work is given appropriate credit. Contents may not be used for commecial purposes, or adapted, remixed, transformed or built upon. (https://creativecommons.org/licenses/by-nc-nd/4.0/)

Georg Thieme Verlag KG
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  • References

  • 1 Rice JA, Carrasco-Medina L, Hodgins DC, Shewen PE. Mannheimia haemolytica and bovine respiratory disease. Anim Health Res Rev 2007; 8: 117-128
    CrossrefPubMedGoogle Scholar
  • 2 Caswell JL. Failure of respiratory defenses in the pathogenesis of bacterial pneumonia of cattle. Vet Pathol 2014; 51: 393-409
    CrossrefPubMedGoogle Scholar
  • 3 Murray GM, OʼNeill RG, More SJ, McElroy MC, Earley B, Cassidy JP. Evolving views on bovine respiratory disease: An appraisal of selected key pathogens – Part 1. Vet J 2016; 217: 95-102
    CrossrefPubMedGoogle Scholar
  • 4 Dabo SM, Taylor JD, Confer AW. Pasteurella multocida and bovine respiratory disease. Anim Health Res Rev 2007; 8: 129-150
    CrossrefPubMedGoogle Scholar
  • 5 Confer AW, Ayalew S. Mannheimia haemolytica in bovine respiratory disease: Immunogens, potential immunogens, and vaccines. Anim Health Res Rev 2018; 19: 79-99
    CrossrefPubMedGoogle Scholar
  • 6 Friend SC, Wilkie BN, Thomson RG, Barnum DA. Bovine pneumonic pasteurellosis: Experimental induction in vaccinated and nonvaccinated calves. Can J Comp Med 1977; 41: 77-83
    PubMedGoogle Scholar
  • 7 de Jong A, Thomas V, Simjee S, Moyaert H, El Garch F, Maher K, Morrissey I, Butty P, Klein U, Marion H, Rigaut D, Vallé M. Antimicrobial susceptibility monitoring of respiratory tract pathogens isolated from diseased cattle and pigs across Europe: The VetPath study. Vet Microbiol 2014; 172: 202-215
    CrossrefPubMedGoogle Scholar
  • 8 Portis E, Lindeman C, Johansen L, Stoltman G. A ten-year (2000–2009) study of antimicrobial susceptibility of bacteria that cause bovine respiratory disease complex–Mannheimia haemolytica, Pasteurella multocida, and Histophilus somni–in the United States and Canada. J Vet Diagn Invest 2012; 24: 932-944
    CrossrefPubMedGoogle Scholar
  • 9 Coetzee JF, Magstadt DR, Sidhu PK, Follett L, Schuler AM, Krull AC, Cooper VL, Engelken TJ, Kleinhenz MD, OʼConnor AM. Association between antimicrobial drug class for treatment and retreatment of bovine respiratory disease (BRD) and frequency of resistant BRD pathogen isolation from veterinary diagnostic laboratory samples. PLoS One 2019; 14: e0219104
    CrossrefPubMedGoogle Scholar
  • 10 Timsit E, Hallewell J, Booker C, Tison N, Amat S, Alexander TW. Prevalence and antimicrobial susceptibility of Mannheimia haemolytica, Pasteurella multocida, and Histophilus somni isolated from the lower respiratory tract of healthy feedlot cattle and those diagnosed with bovine respiratory disease. Vet Microbiol 2017; 208: 118-125
    CrossrefPubMedGoogle Scholar
  • 11 Stanford K, Zaheer R, Klima C, McAllister T, Peters D, Niu YD, Ralston B. Antimicrobial resistance in members of the bacterial bovine respiratory disease complex isolated from lung tissue of cattle mortalities managed with or without the use of antimicrobials. Microorganisms 2020; 8: 288
    CrossrefPubMedGoogle Scholar
  • 12 Ayrle H, Mevissen M, Kaske M, Nathues H, Gruetzner N, Melzig M, Walkenhorst M. Medicinal plants–prophylactic and therapeutic options for gastrointestinal and respiratory diseases in calves and piglets? A systematic review. BMC Vet Res 2016; 12: 89
    CrossrefPubMedGoogle Scholar
  • 13 Bouy M. Ätherische Öle für Wiederkäuer – Bewährte Indikationen und Fallbeispiele aus der französischen Nutztierpraxis. 33. Schweizerische Jahrestagung für Phytotherapie 2018 – Ätherische Öle und ihr therapeutisches Potential. Baden, Switzerland; 2018
    CrossrefGoogle Scholar
  • 14 Brendieck-Worm C, Klarer F, Stöger E. Heilende Kräuter für Tiere: Pflanzliche Hausmittel für Heim- und Nutztiere. 2nd ed.. ed. Bern: Haupt Verlag; 2018
    Google Scholar
  • 15 Reichling J, Schnitzler P, Suschke U, Saller R. Essential oils of aromatic plants with antibacterial, antifungal, antiviral, and cytotoxic properties–an overview. Forsch Komplementmed 2009; 16: 79-90
    PubMedGoogle Scholar
  • 16 Kalemba D, Kunicka A. Antibacterial and antifungal properties of essential oils. Curr Med Chem 2003; 10: 813-829
    CrossrefPubMedGoogle Scholar
  • 17 Amat S, Baines D, Timsit E, Hallewell J, Alexander TW. Essential oils inhibit the bovine respiratory pathogens Mannheimia haemolytica, Pasteurella multocida and Histophilus somni and have limited effects on commensal bacteria and turbinate cells in vitro . J Appl Microbiol 2019; 126: 1668-1682
    CrossrefPubMedGoogle Scholar
  • 18 Amat S, Baines D, Alexander TW. A vapour phase assay for evaluating the antimicrobial activities of essential oils against bovine respiratory bacterial pathogens. Lett Appl Microbiol 2017; 65: 489-495
    CrossrefPubMedGoogle Scholar
  • 19 Salzmann BA. Antimikrobielle In-vitro-Wirkung von acht ätherischen Ölen auf Pasteurella multocida und Mannheimia haemolytica aus Nasentupferproben von Kälbern [Master Thesis]. Bern: Vetsuisse-Faculty; 2019
    PubMedGoogle Scholar
  • 20 Janssen AM, Scheffer JJ, Baerheim Svendsen A. Antimicrobial activity of essential oils: A 1976–1986 literature review. Aspects of the test methods. Planta Med 1987; 53: 395-398
    Article in Thieme ConnectPubMedGoogle Scholar
  • 21 Pankey GA, Sabath LD. Clinical relevance of bacteriostatic versus bactericidal mechanisms of action in the treatment of Gram-positive bacterial infections. Clin Infect Dis 2004; 38: 864-870
    CrossrefPubMedGoogle Scholar
  • 22 Do TKT, Hadji-Minaglou F, Antoniotti S, Fernandez X. Authenticity of essential oils. TrAC Trends Anal Chem 2015; 66: 146-157
    CrossrefPubMedGoogle Scholar
  • 23 Lis-Balchin M, Deans SG, Eaglesham E. Relationship between bioactivity and chemical composition of commercial essential oils. Flavour Fragr J 1998; 13: 98-104
    CrossrefPubMedGoogle Scholar
  • 24 Kissels W, Wu X, Santos RR. Short communication: Interaction of the isomers carvacrol and thymol with the antibiotics doxycycline and tilmicosin: In vitro effects against pathogenic bacteria commonly found in the respiratory tract of calves. J Dairy Sci 2017; 100: 970-974
    CrossrefPubMedGoogle Scholar
  • 25 LeBel G, Vaillancourt K, Bercier P, Grenier D. Antibacterial activity against porcine respiratory bacterial pathogens and in vitro biocompatibility of essential oils. Arch Microbiol 2019; 201: 833-840
    CrossrefPubMedGoogle Scholar
  • 26 Fararh KM, Farid SA, Abd EL-Hamied SS, El-Sharkawy RB. Clinicopathological changes in calves with respiratory diseases after treatment with essential volatile oil and other drugs. BVMJ 2017; 33: 237-247
    PubMedGoogle Scholar
  • 27 Ben-Arye E, Dudai N, Eini A, Torem M, Schiff E, Rakover Y. Treatment of upper respiratory tract infections in primary care: a randomized study using aromatic herbs. Evid Based Complement Alternat Med 2011; 2011: 690346
    CrossrefPubMedGoogle Scholar
  • 28 Cohen BM, Dressler WE. Acute aromatics inhalation modifies the airways. Effects of the common cold. Respiration 1982; 43: 285-293
    CrossrefPubMedGoogle Scholar
  • 29 Wu Q, Yu L, Qiu J, Shen B, Wang D, Soromou LW, Feng H. Linalool attenuates lung inflammation induced by Pasteurella multocida via activating Nrf-2 signaling pathway. Int Immunopharmacol 2014; 21: 456-463
    CrossrefPubMedGoogle Scholar
  • 30 Amat S, Timsit E, Baines D, Yanke J, Alexander TW. Development of bacterial therapeutics against the bovine respiratory pathogen Mannheimia haemolytica . Appl Environ Microbiol 2019; 85: e01359-01419
    CrossrefPubMedGoogle Scholar
  • 31 Becker J, Schüpbach-Regula G, Steiner A, Perreten V, Wüthrich D, Hausherr A, Meylan M. Effects of the novel concept ‘outdoor veal calf’ on antimicrobial use, mortality and weight gain in Switzerland. Prev Vet Med 2020; 176: 104907
    CrossrefPubMedGoogle Scholar
  • 32 Bismarck D, Schneider M, Müller E. Antibakterielle In-vitro-Wirksamkeit ätherischer Öle gegen veterinärmedizinisch relevante Keime klinischer Isolate von Hunden, Katzen und Pferden. Complement Med Res 2017; 24: 153-163
    CrossrefPubMedGoogle Scholar
  • 33 Cermelli C, Fabio A, Fabio G, Quaglio P. Effect of eucalyptus essential oil on respiratory bacteria and viruses. Curr Microbiol 2008; 56: 89-92
    CrossrefPubMedGoogle Scholar
  • 34 CLSI. Performance Standards for antimicrobial Disk and Dilution Susceptibility Tests for Bacteria isolated from Animals. 4th ed. CLSI supplement VET08. Wayne, PA: Clinical and Laboratory Standards Institute; 2018
    Google Scholar
  • 35 R Core Team. R: A language and environment for statistical computing. R Foundation for Statistical Computing. Vienna, Austria. 2021 Accessed November 2, 2021 at: https://www.R-project.org/
    PubMedGoogle Scholar
  • 36 Wickham H. ggplot2: Elegant graphics for data analysis. New York: Springer; 2009
    CrossrefGoogle Scholar
  • 37 Elkin LA, Kay M, Higgins JJ, Wobbrock JO. An Aligned Rank Transform Procedure for Multifactor Contrast Tests. In: Nichols J, Kumar R, Nebeling M. eds. The 34th Annual ACM Symposium on User Interface Software and Technology. New York: ACM; 2021: 754-768
    CrossrefGoogle Scholar
  • 38 Hiller K, Melzig MF. Lexikon der Arzneipflanzen und Drogen. 2nd ed.. ed. Heidelberg: Spektrum Akademischer Verlag; 2010
    CrossrefGoogle Scholar
  • 39 World Health Organization. WHO monographs on selected medicinal plants, Volume 2. Geneva: World Health Organization; 2002
    Google Scholar
  • 40 European Scientific Cooperative on Phytotherapy. ESCOP Monographs Online Series, Caryophylli Aetheroleum – clove oil. Exeter: European Scientific Cooperative on Phytotherapy; 2014
    Google Scholar
  • 41 Nath SC, Saha BN, Bordoloi DN, Mathur RK, Leclercq PA. The chemical composition of the essential oil of Cymbopogon flexuosus (Steud) Wats. Growing in Northeast India. J Essent Oil Res 1994; 6: 85-87
    CrossrefPubMedGoogle Scholar