Planta Med
DOI: 10.1055/a-2162-4018
Original Papers

Impact of Phylogenetically Diverse Bacterial Endophytes of Bergenia pacumbis on Bergenin Production in the Plant Cell Suspension Cultures[ # ]

Department of Pharmaceutical Sciences, Division of Pharmacognosy, University of Vienna, Vienna, Austria
Martina Oberhofer
Department of Pharmaceutical Sciences, Division of Pharmacognosy, University of Vienna, Vienna, Austria
Stefan Steinbrecher
Department of Pharmaceutical Sciences, Division of Pharmacognosy, University of Vienna, Vienna, Austria
Sergey B. Zotchev
Department of Pharmaceutical Sciences, Division of Pharmacognosy, University of Vienna, Vienna, Austria
› Author Affiliations
This work was funded by the University of Vienna.


Plant in vitro cultures are potential sources for secondary metabolites. However, low productivity is often a major drawback for industrial application. Elicitation is an important strategy to improve product formation in vitro. In this context, endophytes are of special interest as biotic elicitors due to their possible interaction with the metabolism of the host plant. A total of 128 bacterial endophytes were isolated from the medicinal plant Bergenia pacumbis and taxonomically classified using 16S rRNA gene sequencing. Five strains belonging to different genera were grown in lysogeny broth and tryptic soy broth medium and cells as well as spent media were used as elicitors in cell suspension cultures of B. pacumbis. Production of the main bioactive compound bergenin was enhanced 3-fold (964 µg/g) after treatment with cells of Moraxella sp. or spent tryptic soy broth medium of Micrococcus sp. These results indicate that elicitation of plant cell suspension cultures with endophytic bacteria is a promising strategy for enhancing the production of desired plant metabolites.

# This work is dedicated to Professors Rudolf Bauer, Chlodwig Franz, Brigitte Kopp, and Hermann Stuppner for their invaluable contributions and commitment to Austrian pharmacognosy.

Publication History

Received: 19 June 2023

Accepted after revision: 23 August 2023

Article published online:
06 September 2023

© 2023. Thieme. All rights reserved.

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