Planta Medica, Table of Contents Planta Med 2024; 90(07/08): 651-657DOI: 10.1055/a-2162-4018 Natural Product Chemistry and Analytical Studies Original Papers Impact of Phylogenetically Diverse Bacterial Endophytes of Bergenia pacumbis on Bergenin Production in the Plant Cell Suspension Cultures[ # ] Authors Author Affiliations Christoph Wawrosch Department of Pharmaceutical Sciences, Division of Pharmacognosy, University of Vienna, Vienna, Austria Martina Oberhofer Department of Pharmaceutical Sciences, Division of Pharmacognosy, University of Vienna, Vienna, Austria Stefan Steinbrecher Department of Pharmaceutical Sciences, Division of Pharmacognosy, University of Vienna, Vienna, Austria Sergey B. Zotchev Department of Pharmaceutical Sciences, Division of Pharmacognosy, University of Vienna, Vienna, Austria Recommend Article Abstract Buy Article(opens in new window) Abstract Plant in vitro cultures are potential sources for secondary metabolites. However, low productivity is often a major drawback for industrial application. Elicitation is an important strategy to improve product formation in vitro. In this context, endophytes are of special interest as biotic elicitors due to their possible interaction with the metabolism of the host plant. A total of 128 bacterial endophytes were isolated from the medicinal plant Bergenia pacumbis and taxonomically classified using 16S rRNA gene sequencing. Five strains belonging to different genera were grown in lysogeny broth and tryptic soy broth medium and cells as well as spent media were used as elicitors in cell suspension cultures of B. pacumbis. Production of the main bioactive compound bergenin was enhanced 3-fold (964 µg/g) after treatment with cells of Moraxella sp. or spent tryptic soy broth medium of Micrococcus sp. These results indicate that elicitation of plant cell suspension cultures with endophytic bacteria is a promising strategy for enhancing the production of desired plant metabolites. Key words Key words Bergenia pacumbis - Saxifragaceae - cell suspension culture - bergenin - endophytes - elicitation Full Text References References 1 Hardy K. Paleomedicine and the evolutionary context of medicinal plant use. Rev Bras Farmacog 2021; 31: 1-15 2 Khan MSA, Ahmad I. Herbal Medicine: Current Trends and Future Prospects. In: Khan MSA, Ahmad I, Chattopadhyay D. eds. New Look to Phytomedicine. London: Academic Press; 2019: 3-13 3 Noviana E, Indrayanto G, Rohman A. Advances in fingerprint analysis for standardization and quality control of herbal medicines. 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