Planta Med 2015; 81 - PX48
DOI: 10.1055/s-0035-1556492

Investigation of the antiproliferative activity of Linociera ramiflora and its isolates

PA Benatrehina 1, L Pan 1, CB Naman 1, HB Chai 1, TN Ninh 2, DD Soejarto 3, 4, LH Rakotondraibe 1, AD Kinghorn 1
  • 1Division of Medicinal Chemistry and Pharmacognosy, College of Pharmacy, The Ohio State University, Columbus, OH 43210, USA
  • 2Institute of Ecology and Biological Resources, Vietnam Academy of Science and Technology, Hanoi, Vietnam
  • 3College of Pharmacy, University of Illinois at Chicago, Chicago, IL 60612, USA
  • 4Science and Technology, Field Museum, 1400 S. Lakeshore Dr., Chicago, IL 60605, USA

Following the primary screening of Vietnamese plant extracts against the HT-29 human colon cancer cell line assay, the branch sample of Linociera cf. ramiflora (Roxb.) Wall. (Oleaceae) (sample A06576; voucher specimen DDS 14374) was selected as a candidate for further study. The methanol extract of this plant sample was subjected to a bioactivity-guided fractionation aiming at the isolation of potential anticancer agents. Liquid-liquid partitions of this extract led to an active chloroform fraction (IC50= 7.7 µg/mL). Further separation of this fraction through successive column chromatography afforded numerous active sub-fractions, from which several new and known lignans and arylglycerol-substituted lignan derivatives together with some aromatic compounds were isolated. The structure determination as well as the antiproliferative and quinone reductase inducting activities of these compounds will be described.