Oligonucleotide Arrays from Aldehyde-Bearing Glass with Coated Background

Kendra L. Dombi; Niels Griesang; Clemens Richert

doi:10.1055/s-2002-25758

SPECIALTOPIC

Oligonucleotide Arrays from Aldehyde-Bearing Glass with Coated Background

Kendra L. Dombi, Niels Griesang, Clemens Richert*
Department of Chemistry, University of Constance, Fach M 709, 78457 Konstanz, Germany
Fax: +49(7531)884573; e-Mail: Clemens.Richert@uni-konstanz.de;

Abstract

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Abstract

Presented here is a method for preparing small DNA arrays on aldehyde-bearing glass slides. Immobilization involves reductive amination and employs oligonucleotides with 3′-terminal lysine residues, obtained in high yield from solid phase syntheses. Spot patterns are produced by protecting selected areas of the aldehyde slides with wax, coating the free surface with a methyl triethylene glycol derivative, and removing the wax with dichloromethane. The DNA arrays give better signal to noise ratios in hybridization experiments than slides without passified background.

Key words

oligonucleotides - Schiff bases - solid phase synthesis - DNA - molecular recognition

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