Abstract
Cortex Moutan (root bark of Paeonia suffruticosa Andrew) and Radix Salviae miltiorrhizae (root and rhizome of Salvia miltiorrhiza Bunge) are two herbs widely used in traditional Chinese medicine (TCM) to treat cerebrovascular
and cardiovascular diseases. In clinical practice, these two herbs are prescribed
together. Studies on the pharmacokinetic interaction between the active constituents
of these two herbs (paeonol and danshensu, respectively) can provide substantial foundation
for understanding its mechanism and empirical evidence to support the clinical practice.
A simple and sensitive high-performance liquid chromatographic (HPLC) method coupled
with ultraviolet detector was developed for determination of paeonol in plasma and
different tissues (heart, liver, spleen, lung, kidney, and brain) of male Sprague-Dawley
rats. When co-administering danshensu, the peak plasma concentration of paeonol was
decreased (p < 0.01), the mean residence time (MRT) was prolonged (p < 0.001), the
volume of distribution (Vd/F) was increased (p < 0.001), and the concentrations of
paeonol in heart, brain, and lung were dramatically increased (p < 0.01 or p < 0.001),
compared with these values for rats administered paeonol alone. The results showed
that the co-administration of danshensu could alter pharmacokinetic fate and tissue
distribution of paeonol in rats, especially in heart and brain, providing substantial
foundation for the investigation of the impact of danshensu on paeonol in clinical
applications.
Key words
paeonol - Danshensu - HPLC - pharmacokinetics - tissue distribution - pharmacokinetic
interaction
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Prof. Siwang Wang
Institute of Materia Medica, School of Pharmacy
Fourth Military Medical University
169 West Changle Road
710032 Xi'an
People's Republic of China
Telefon: +86 29 8477 47 48
Fax: +86 29 8322 47 90
eMail: wangsiw@hotmail.com
Associate Prof. Bangle Zhang
Department of Pharmaceutics, School of Pharmacy
Fourth Military Medical University
169 West Changle Road
710032 Xi'an
People's Republic of China
Telefon: +86 29 8477 45 56
Fax: +86 29 8477 45 52
eMail: blezhang@hotmail.com