Three New Cycloartenol Glycosides from the Roots of Cimicifuga simplex

Abstract

Three new cycloartenol triterpene saponins, 3β,16α-dihydroxy-12-acetoxy-16,22-cyclo-23-ketone-24R,25-epoxy-cycloartane-7-ene 3-O-β-D-galactopyranoside (1), 24-O-hydroxy-7,8-didehydrohydroshengmanol 3-O-β-D-galactopyranoside (2), and 24-epi-24-O-hydroxy-7,8-didehydrohydroshengmanol 3-O-β-D-galactopyranoside (3), were isolated from the ethyl acetate soluble fraction of Cimicifuga simplex. Their structures were established by detailed spectroscopic analysis, including extensive 2D-NMR data. This is the first time that a 16,22-cyclo type glycosidesaponin from a Cimicifuga species was reported. The immunosuppressive activities of the new compounds were evaluated by a ConA-stimulated T splenocyte proliferation assay in vitro.

Cimicifuga simplex Wormsk - Ranunculaceae - cycloartane glycosides - immunosuppressive activity

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