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Planta Med 2013; 79(11): 924-932
DOI: 10.1055/s-0032-1328718
Biological and Pharmacological Activity
Original Papers
Georg Thieme Verlag KG Stuttgart · New York

Effects of an Aqueous Extract from Leaves of Ligustrum vulgare on Mediators of Inflammation in a Human Neutrophils Model

Monika E. Czerwińska
Department of Pharmacognosy and Molecular Basis of Phytotherapy, Medical University of Warsaw, Warsaw, Poland
,
Sebastian Granica
Department of Pharmacognosy and Molecular Basis of Phytotherapy, Medical University of Warsaw, Warsaw, Poland
,
Anna K. Kiss
Department of Pharmacognosy and Molecular Basis of Phytotherapy, Medical University of Warsaw, Warsaw, Poland
› Author Affiliations
Further Information

Publication History

received 11 February 2013
revised 17 May 2013

accepted 20 May 2013

Publication Date:
03 July 2013 (online)

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Abstract

Leaves of Ligustrum vulgare (common privet) have been used for treatment of oropharyngeal inflammations or as antirheumatic, diuretic, and hypotensive agents in folk medicine in southern Europe. Taking into account that neutrophils are involved in the inflammation, the aim of the study was to determine the effect of an aqueous extract prepared from leaves of Ligustrum vulgare on neutrophil functions. The extract was characterized by the HPLC-DAD-MSn method. The inhibition of reactive oxygen species production by formyl-met-leu-phenylalanine- or phorbol 12-myristate 13-acetate-stimulated neutrophils was determined using luminol- or lucigenin-dependent chemiluminescence. The effect on myeloperoxidase, metalloproteinase 9, and interleukin 8 production by neutrophils was measured by an enzyme-linked immunosorbent assay. Neutrophil elastase release was established spectrophotometrically. The expression of adhesion molecules on neutrophils was analyzed with flow cytometry. The main compounds detected were flavonoids, phenylpropanoids, hydroxycinnamates, and secoiridoids. The inhibition of oxidative burst by the extract was comparable in both stimuli models (formyl-met-leu-phenylalanine: IC50 = 18.2 ± 4.0 µg/mL; phorbol 12-myristate 13-acetate: IC50 = 19.8 ± 3.0 µg/mL). The extract in the concentration range of 5–50 µg/mL inhibited neutrophil elastase release by 23.9–34.1 % and myeloperoxidase release by 24.2–37.4 %. The inhibitory effect on metalloproteinase 9 and interleukin 8 production was around 20 %. The extract in the highest concentration modulated the expression of L-selectin and β 2 integrin. Our results partly support the traditional use of common privet leaves as an anti-inflammatory agent.

Key words

Ligustrum vulgare L. - Oleaceae - neutrophil oxidative burst - myeloperoxidase - neutrophil elastase - HPLC-DAD-MS
 

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