Comparing Short-Duration Electro-Oculograms with and without Mydriasis in Healthy Subjects

Abstract

Background: In order to obtain artifact-free electro-oculogram recordings the subjectʼs cooperation is necessary. The aim of our study is to evaluate the recording characteristics of short-duration EOG and to compare the effect of mydriasis on electro-oculogram recordings in a cohort of controls. Patients and Methods: Electro-oculogram recordings were performed on a light-emitting diode stimulus screen using a RETI-port gamma plus² system (RETIscan™, Roland Consult). Fast oscillations were set at 1.5 sec (6 cycles; total duration 75 sec).The dark phase included: pre-adaptation (6 min), alternate fixation (4 min), fixation-rest (20 sec), 100 sweeps. The light phase included: light adaptation (4 min), alternate fixation (10 min), fixation-rest (20 sec), 250 sweeps. The amplifier band pass was filtered at 0.1 ÷ 50 Hz. The background illumination in mydriasis was 100 cd/m² and in miosis – 450 cd/m². Results: A total of 55 controls participated and were divided into three age groups [number; mean (years, y); ± SD]: group 1: 18–20 years (19; 19.49 years; ± 0.89); group 2: 20–40 years (18; 27.91 years; ± 5.39) and group 3: 40–60 years (18; 48.66 years; ± 4.00). The Arden ratio, dark-trough and light-peak did not differ between recordings with or without mydriasis (p = 0.914; p = 0.880; p = 0.680, linear mixed-effects model). The age did not influence the Arden ratio, dark-trough, light-peak (p = 0.206; p = 0.112; p = 0.155). Arden ratio, dark-trough, light-peak were comparable between tested eyes (p = 0.934; p = 0.193; p = 0.270). Conclusions: Short-duration electro-oculograms allow successful recording, furthermore, the application of mydriasis does not influence the quality of the recording.

Zusammenfassung

Hintergrund: Um Artefakt-freie Elektrooculogramm Aufnahmen zu erhalten, ist die Kooperation des Patienten nötig. Das Ziel unserer Studie war es, die Merkmale von kurzzeitigen EOG Aufnahmen zu bewerten und den Effekt der Mydriase zu evaluieren. Patienten und Methoden: Elektrooculogramm Aufnahmen wurden auf einen Leuchtdioden Stimulus Bildschirm bei Gebrauch eines RETI-port gamma plus² System (RETIscanTM, Roland Consult) gewonnen. Schnelle Schwingungen waren auf 1.5 sek (6 Zyklen; Totale Dauer 75 sek) eingestellt. Dunkelphase inbegriffen: Präadaptation (6 min), alternierte Fixation (4 min), Fixationsruhe (20 sek), 100 Sweeps. Lichtphase inbegriffen: Licht Adaptation (4 min), alternierte Fixation (10 min), Fixationsruhe (20 sek), 250 Sweeps. Im Verstärker wurde ein Bandbreitenfilter für 0.1 ÷ 50 Hz eingestellt. Die Hintergrundbeleuchtung mit Mydriase war 100 cd/m² und ohne Mydriase 450 cd/m². Ergebnisse: 55 gesunde Probanden in 3 Altersgruppen nahmen teil (Anzahl; mittleres Alter [Jahre, y]; ± SD): Gruppe 1: 18–20 y (19; 19,49 y; ± 0,89); Gruppe 2: 20–40 y (18; 27,91 y; ± 5,39) und Gruppe 3: 40–60 y (18; 48,66 y; ± 4,00). Arden-Quotient, Dunkeltalwerte, Hellgipfelwerte unterschieden sich nicht zwischen den Aufnahmen mit und ohne Mydriase (p = 0,914; p = 0,880; p = 0,680, Lineare Regression). Das Alter hatte keinen Einfluss auf den Arden-Quotient, Dunkeltalwerte und Hellgipfelwerte (p = 0,206; p = 0,112; p = 0,155). Arden-Quotient, Dunkeltalwerte und Hellgipfelwerte waren vergleichbar unter den getesteten Augen (p = 0,934; p = 0,193; p = 0,270). Schlussfolgerung: Kurzzeit-Elektrooculogramms können mit guten Resultaten erhoben werden und zeigen keine Beeinträchtigung durch eine Mydriase.

Schlüsselwörter

Arden-Quotient - ISCEV-Standard - Elektrooculogramm - Pigmentepithelium - Mydriase

Key words

Arden ratio - ISCEV standards - electro-oculogram (EOG) - retinal pigment epithelium (RPE) - mydriasis

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