◊ Contributed equally to this work
Key words radicals - protecting-group-free synthesis - atom-transfer radical addition - ATRA
- boschnialactone - lycorane
Although protecting groups are crucial in synthesis, as Philip Kocieński has stated
in his classic book,[1 ] the truth is that they have a tremendous ecological and economic cost that sometimes
is not worth the expense. Consequently, many synthetic strategies to circumvent protecting
groups have been reported.[2 ] In this regard, and due to the neutral character of free radicals, these reactive
intermediates are the ideal partners in protecting-group-free (PGF) synthesis. In
particular, atom transfer radical addition (ATRA) can provide suitable intermediates
that can be transformed into useful molecules for the construction of complex natural
products. For instance, either the transferred atom or group (halogen, usually) provides
a new starting point for additional radical or ionic reactions. Accordingly, in recent
years, our group has developed new radical–ionic sequences for the preparation of
epoxides,[3 ] lactams,[4 ] 1,4-dicarbonyl compounds,[5 ] and iodolactones,[6 ] showcasing the usefulness of this strategy (Scheme [1 ]). Now, by using these synthetic strategies, the total synthesis of (–)-boschnialactone
(1 ) and (±)-γ-lycorane (2 ) is reported.
Scheme 1 Preparation of 1,4-dicarbonyl compounds and γ-iodolactones through ATRA–ionic sequences
Boschnialactone (1 ), an iridoid monoterpene lactone isolated from Boschniakia rossica by Sakan and co-workers,[7 ] exhibits interesting insecticidal activities. A number of racemic[8 ] and asymmetric[9 ] syntheses of 1 have been reported, albeit to the best of our knowledge, with the exception of Nangia’s
approach,[9d ] all employing protecting groups. On the other hand, γ-lycorane is a lycorine-type
natural product belonging to the Amaryllidaceae alkaloids.[10 ] Although many of these alkaloids possess interesting biological activities, γ-lycorane
apparently does not exhibit useful pharmaceutical properties. However, it is a popular
synthetic target for structural reasons, and several racemic[11 ] and asymmetric[12 ] syntheses have been reported (Figure [1 ]).
Figure 1 Representative iridoid monoterpenes and lycorine-type alkaloids
The strategy for the synthesis of compounds 1 and 2 is shown in Scheme [2 ]. In both cases, a radical–ionic sequence would be the key step. For boschnialactone
(1 ), a rapid assembly was envisioned by using our lactonization protocol[6 ] from iodoacetic acid and allylic alcohol 8 , which would be prepared by a PGF route from (R )-citronellal (7 ). The advanced intermediate 11 for the synthesis of γ-lycorane (2 ) can be prepared from a radical–ionic sequence[5 ] between enol acetate 10 and iodoacetonitrile. The former compound would be obtained in three steps from cyclohexene
oxide (9 ) in a PGF fashion.
Scheme 2 Strategy for the synthesis of 1 and 2
The synthesis of compound 1 commenced with the preparation of the respective radical acceptor 8 (Scheme [3 ]). Several described routes were studied for obtaining alcohol 8 , in both racemic and enantiopure forms, however the best results were obtained by
applying slightly modified conditions to those reported by Chavez and Jacobsen[9e ] for aldehyde 13 . Methylenation of (R )-citronellal afforded compound 12 in 98% yield; then, RCM reaction with 10 mol% second-generation Grubbs catalyst rendered
aldehyde 13 , which was directly reduced with DIBAL-H to the desired alcohol 8 in 60% yield for two steps. With radical acceptor 8 in hand, we proceeded to apply the key radical–ionic sequence. Treatment of a refluxing
solution of 8 and iodoacetic acid (14 , 2.4 equiv) in 1,2-dichloroethane (1,2-DCE) with lauroyl peroxide (DLP) generated
unstable iodolactone 15 , which was treated in one pot with Bu3 SnH and 1,1′-azobis(cyclohexanecarbonitrile) (ACHN) after switching the solvent from
1,2-DCE to benzene. The crude reaction product revealed the presence of an inseparable
7:1 mixture of stereoisomers. The major isomer corresponded to (–)-boschnialactone
(1 ) in 30% yield, whose chemical and spectroscopic data matched those reported previously.[9e ]
Scheme 3 PGF total synthesis of (–)-boschnialactone (1 )
A plausible explanation for the observed cis selectivity is depicted in Scheme [4 ]; low-energy conformer 8b is attacked by radical R• , derived from iodoacetic acid, at the bottom face of the envelope conformation, in
order to avoid steric interactions with C4. This gives rise to intermediate 16 , in which hydrogen-atom transfer by Bu3 SnH is carried out anti to substituents placed at C1 and C3, affording intermediate 17 with the desired stereochemistry. Subsequent in situ iodoacetic acid mediated lactonization furnishes the observed boschnialactone (1 ). Although attack of radical R• at conformer 8a is also possible, lactone 1 was the only isolated compound, and these results are consistent with the observations
by Field and Gallagher[13 ] for the hydroboration of 1,5-dimethylcyclopentene.
Scheme 4 Plausible explanation for the stereoselectivity for lactone 1
The preparation of enol acetate 10 , the radical acceptor for the synthesis of γ-lycorane (2 ), is depicted in Scheme [5 ]. Known ketone 18 ,[11g ] prepared in two steps from cyclohexene oxide (9 ), was converted into enol acetate 10 by enolate formation with LDA and trapping with Ac2 O. It is worth mentioning that the acetate group is not a protecting group but is
a way to switch the reactivity of the ketone moiety and to convert it into the required
radical acceptor. With compound 10 in hand, the first approach to γ-lycorane was planned with the radical addition of
ethyl iodoacetate to assemble 19 . However, the latter compound was isolated as an inseparable mixture of diastereoisomers
(ca. 2:1) in low yield (26%); therefore, an adjustment of the oxidation level of the
ester group was required before the introduction of the nitrogen atom. Reduction of
both the ester and the ketone group followed by oxidation provided keto aldehyde 21 , which was submitted to reductive amination with ammonium acetate and sodium cyanoborohydride.
Unexpectedly, the only recovered product was pyrrole 22 in 80% yield, even under different reaction conditions.
Scheme 5 Formal synthesis of γ-lycorane (2 ) via pyrrole 22
Although pyrrole 22 is known[11k ] as a precursor of γ-lycorane (2 ), we looked for the possibility of performing a more straightforward and efficient
route. In this regard, we anticipated that the addition of iodoacetonitrile to 10 would provide a compound with the correct carbon chain and the required nitrogen
atom, which would eventually make part of the alkaloid skeleton through an intramolecular
reductive amination. Compound 10 was then subjected to the previously employed conditions [DLP (1.2 equiv), added
portionwise in refluxing 1,2-DCE] to afford keto nitrile 23 in 84% yield as a separable mixture of 1,3-cis (23a , 81%, major) and 1,3-trans (23b , 3%, minor) diastereoisomers. Although the stereochemistry of these compounds could
not be established at this point, the further transformation of 23a into γ-lycorane (see Scheme [7 ]) allowed confirmation of the 1,3-cis relationship. Even though 23a is the more stable stereoisomer due to the equatorial disposition of both substituents,
the major compound observed by TLC during the reaction and by analysis of the crude
1 H NMR spectrum was 23b . However, after column chromatography (silica gel, hexane/EtOAc/NEt3 ), the only recovered product was 23a , whereas 23b could only be isolated, albeit in low yield (20%), when triethylamine was excluded
from the eluent system. To confirm the assumption that 23b was the major product which was then converted into the more stable 23a during column chromatography, a solution of 23b in hexane/ethyl acetate was stirred at room temperature with silica gel and NEt3 , and its complete transformation into 23a was observed within 2 hours. In contrast, 23b treated solely with either silica gel or NEt3 did not show any change after several hours. These observations allowed us to conclude
that 23b is the major isomer formed in the reaction, but epimerization during column chromatography
afforded the 1,3-cis compound 23a . The stereoselectivity for the radical addition to enol acetate 10 can be explained according to the well-known conformation of cyclohexenes. Accordingly,
compound 10 has the preferred half-chair conformation 10a , with the aryl substituent in the equatorial position. Radical R• can thus approach from the top (path a ) or the bottom (path b ) face, leading in the former case to a less stable twisted boat conformation 10b , which would eventually form 1,3-cis compound 23a . On the contrary, attack of the radical from the bottom face (path b ) would force the product to adopt an alternate, more stable chair conformation 10c which, after transformation into the carbonyl compound, gives the 1,3-trans compound 23b (Scheme [6 ]).
Scheme 6 Plausible explanation for the stereoselectivity for 23a /23b
With keto nitrile 23a in hand, the next step consisted of the transformation of the nitrile moiety into
a primary amine followed by an intramolecular reductive amination. A number of methods
were tested, such as NaBH4 /NiCl2 , LiAlH4 , H2 /Raney Ni, H2 /Pd-C; however, incomplete reactions or a complex mixture of products was observed.
After considerable experimentation, it was found that when 23a was hydrogenated at 250 psi in the presence of PtO2 , both the nitrile reduction and the reductive amination occurred, and the formation
of 24 was accomplished in 90% yield. The spectroscopic data of 24 fully matched those reported,[11 ] and according to Bates and co-workers[11k ] 24 can be directly transformed into γ-lycorane (2 ) through a Pictet–Spengler reaction (Scheme [7 ]).
Scheme 7 Final steps for the formal synthesis of γ-lycorane (2 )
In conclusion, we have reported two free-radical approaches to structurally different
natural products: an iridoid, with a six-membered lactone as the main structure, and
a lycorine-type alkaloid. Both syntheses rely on the use of a radical–ionic sequence
as the key step, and because of the known tolerance of free radicals to unprotected
functional groups, these steps were performed in a PGF fashion.
All operations were carried out under an inert atmosphere of nitrogen or argon gas
using standard techniques. Anhydrous THF was obtained by distillation over sodium
and benzophenone under an inert atmosphere. Column chromatography was performed using
70–230 mesh silica gel. All reagents and solvents were obtained from commercial suppliers
and used without further purification. Melting points were obtained on a Melt-Temp
II apparatus and are uncorrected. NMR spectra were measured with a JEOL Eclipse +300
or a Bruker Ascend (500 MHz) spectrometer, using CDCl3 as solvent. Chemical shifts are in ppm (δ), relative to TMS. MS (DART) spectra were
obtained on a JEOL DART AccuTOF JMS-T100CC instrument; signal values are expressed
in mass/charge units (m /z ).
3,7-Dimethyl-2-methyleneoct-6-enal (12)
3,7-Dimethyl-2-methyleneoct-6-enal (12)
To a solution of (R )-citronellal (7 ; 1 g, 6.48 mmol) and 37% aqueous formaldehyde solution (0.4 mL, 14.2 mmol) in i -PrOH (1.5 mL) at r.t. were slowly added propionic acid (0.12 mL, 1.62 mmol) and pyrrolidine
(0.14 mL, 1.62 mmol). Then, the mixture was heated at 45 °C for 3 h until the starting
material was completely consumed (monitored by TLC). The reaction was quenched with
H2 O (10 mL) and the mixture extracted with DCM (3 × 15 mL). The combined organic layers
were washed with brine (10 mL), dried with Na2 SO4 , and concentrated in vacuo . The residue was purified by flash chromatography (silica gel, hexane) to afford
aldehyde 12 as a colorless oil; yield: 1.06 g (98%). The spectroscopic data matched those reported
by Pihko.[14 ]
[α]D
25 +5.30 (c 1, DCM).
1 H NMR (300 MHz, CDCl3 ): δ = 9.49 (s, 1 H), 6.19 (s, 1 H), 5.95 (s, 1 H), 5.04 (tt, J = 1.2, 7.2 Hz, 1 H), 2.67 (ddd, J = 13.8, 13.8, 6.9 Hz, 1 H), 1.96–1.85 (m, 2 H), 1.63 (s, 3 H), 1.53 (s, 3 H), 1.56–1.44
(m, 1 H), 1.40–1.31 (m, 1 H), 1.03 (d, J = 6.8 Hz, 3 H).
13 C NMR (75 MHz, CDCl3 ): δ = 194.6, 155.5, 133.1, 131.7, 124.2, 35.6, 31.0, 25.8, 25.7, 19.6, 17.7.
(5-Methylcyclopent-1-en-1-yl)methanol (8)
(5-Methylcyclopent-1-en-1-yl)methanol (8)
A solution of aldehyde 12 (0.3 g, 1.8 mmol) in DCM (7 mL) under nitrogen atmosphere was transferred via cannula to a flask containing second-generation Grubbs catalyst (0.1 g, 0.12 mmol),
followed by a second portion of DCM (7 mL) at r.t. The solution was heated at reflux
under nitrogen for 24 h; then, the reaction mixture was filtered through a short pad
of silica gel and washed with anhydrous DCM (15 mL) to remove the remaining solids
of the Grubbs catalyst. The crude product solution was used in the next reaction without
further purification due to the high volatility of aldehyde 13 .
To a solution of the crude aldehyde in DCM (29 mL) at 0 °C under nitrogen atmosphere
was added 1 M DIBAL-H in DCM (3.6 mL, 3.6 mmol). The ice bath was removed, and the
mixture was stirred for a further 40 min at r.t. The reaction was quenched with a
saturated solution of Rochelle salt (5 mL) and the mixture stirred until a white jelly
solid was formed. The reaction mixture was extracted with DCM (3 × 15 mL) and the
combined organic layers were dried over Na2 SO4 . Removal of the solvent in vacuo yielded the crude alcohol which was purified by column chromatography (silica gel,
DCM) to afford alcohol 8 as a pale yellow oil; yield: 0.121 g (60%, two steps).
[α]D
25 –7.14 (c 1, DCM).
1 H NMR (300 MHz, CDCl3 ): δ = 5.59–5.57 (m, 1 H), 4.21 (d, J = 13.8 Hz, 1 H), 4.14 (d, J = 13.8 Hz, 1 H), 2.78–2.66 (m, 1 H), 2.39–2.23 (m, 2 H), 2.19–2.08 (m, 1 H), 1.59
(brs, 1 H), 1.49–1.39 (m, 1 H), 1.03 (d, J = 13.8 Hz, 3 H).
13 C NMR (75 MHz, CDCl3 ): δ = 148.5, 125.3, 60.9, 39.6, 33.1, 30.7, 19.4.
HRMS (DART): m /z [M – OH]+ calcd for C7 H12 O: 95.08553; found: 95.08599.
(–)-Boschnialactone (1)
A solution of allylic alcohol 8 (0.3 g, 2.6 mmol) and iodoacetic acid (14 ; 1.15 g, 6.2 mmol) in 1,2-DCE (15 mL) was heated at reflux under nitrogen for 10
min. Then, DLP (20 mol%) was added every 1.5 h until the starting material was completely
consumed (4 additions, 80 mol%; monitored by TLC). Then, the solvent was evaporated
under reduced pressure and the residue dissolved in benzene (15 mL). To this solution,
ACHN (0.87 g, 1 mmol) and Bu3 SnH (0.8 mL, 2.17 mmol) were sequentially added. The resulting mixture was heated
at reflux under nitrogen for 2 h. When the starting material was completely consumed,
the solvent was removed in vacuo , and the residue was purified by flash column chromatography (hexane/EtOAc, 30:1)
to give 1 as an inseparable 7:1 mixture of diastereoisomers as a white solid; yield: 0.123
g (30%); mp 50–51 °C (Lit.[9e ] 55–56 °C). The spectroscopic properties were identical in all aspects to those reported
previously.[9e ]
[α]D
25 –16.8 (c 1, DCM).
1 H NMR (400 MHz, CDCl3 ): δ = 4.21 (dd, J = 5.5, 11.4 Hz, 1 H), 4.11 (dd, J = 9.2, 11.7 Hz, 1 H), 2.66–2.54 (m, 2 H), 2.46–2.37 (m, 1 H), 2.35–2.25 (m, 1 H),
2.22–2.10 (m, 1 H), 1.94–1.79 (m, 2 H), 1.53–1.45 (m, 1 H), 1.39–1.29 (m, 1 H), 1.02
(d, J = 6.9 Hz, 3 H).
13 C NMR (125 MHz, CDCl3 ): δ = 174.1, 67.7, 39.8, 37.5, 35.2, 35.0, 33.0, 32.9, 14.8.
HRMS (DART): m /z [M + H]+ calcd for C9 H14 O2 : 155.10720; found: 155.10790.
6-(Benzo[d ][1,3]dioxol-5-yl)cyclohex-1-en-1-yl Acetate (10)
6-(Benzo[d ][1,3]dioxol-5-yl)cyclohex-1-en-1-yl Acetate (10)
n -BuLi (2.5 M in hexanes, 1.46 mL, 3.67 mmol) was slowly added to a solution of diisopropylamine
(0.54 mL, 3.85 mmol) in THF (10.5 mL) at 0 °C. The resulting solution was cooled at
–78 °C and stirred for 30 min. Then, a solution of ketone 18 (400 mg, 1.83 mmol) in THF (8 mL) was transferred to the reaction flask, and the
mixture was stirred for 30 min; then, the reaction was quenched with acetic anhydride
(0.612 mL, 5.49 mmol) and the mixture allowed to stir for a further 30 min. The solvent
was evaporated under reduced pressure, and the residue was partitioned between H2 O and EtOAc, and extracted with EtOAc (3 × 15 mL). The combined organic layers were
dried over Na2 SO4 , filtered, and concentrated in vacuo . Purification by column chromatography (silica gel, hexane/EtOAc/NEt3 , 15:1:0.1) afforded compound 10 as a white solid; yield: 405 mg (85%); mp 50–53 °C.
1 H NMR (300 MHz, CDCl3 ): δ = 6.73–6.63 (m, 3 H), 5.90 (s, 2 H), 5.58 (t, J = 3.6 Hz 1 H), 3.67–3.60 (m, 1 H), 2.23–2.16 (m, 2 H), 2.12–2.01 (m, 1 H), 1.85 (s,
3 H), 1.72–1.55 (m, 3 H).
13 C NMR (75 MHz, CDCl3 ): δ = 169.4, 148.6, 147.6, 146.1, 136.5, 121.4, 116.6, 108.5, 108.0, 100.9, 43.0,
33.0, 24.0, 21.0, 19.3.
HRMS (DART): m /z [M + H]+ calcd for C15 H16 O4 : 261.11268; found: 261.11270.
Ethyl 2-(3-(Benzo[d ][1,3]dioxol-5-yl)-2-oxocyclohexyl)acetate (19)
Ethyl 2-(3-(Benzo[d ][1,3]dioxol-5-yl)-2-oxocyclohexyl)acetate (19)
A solution of enol acetate 10 (150 mg, 0.58 mmol) and ethyl iodoacetate (204 μL, 1.73 mmol) in 1,2-DCE (5.8 mL)
was refluxed under nitrogen for 10 min. Then, DLP was added portionwise (69.4 mg,
0.174 mmol) every hour until the complete consumption of starting material (monitored
by TLC). The reaction mixture was concentrated under reduced pressure and the residue
purified by column chromatography (silica gel, hexane/EtOAc/NEt3 , 20:1:0.1) to afford compound 19 as a mixture of diastereoisomers; yield: 46 mg (26%). Only the cis stereoisomer could be isolated as a single diastereoisomer for characterization purposes.
The spectroscopic data matched those reported by Zhou and co-workers.[12g ]
White solid; mp 83–85 °C.
1 H NMR (300 MHz, CDCl3 ): δ = 6.81–6.69 (m, 3 H), 5.95 (s, 2 H), 4.12 (q, J = 7.1 Hz, 2 H), 3.73 (t, J = 4.6 Hz, 1 H), 3.00 (m, 1 H), 2.75 (dd, J = 7.8, 16.4 Hz, 1 H), 2.54–2.45 (m, 1 H), 2.35 (t, J = 7.5 Hz, 2 H), 2.20 (dd, J = 6.0, 16.5 Hz, J = 7.7 Hz, 1 H), 2.10–1.99 (m, 2 H), 1.81–1.70 (m, 1 H), 1.24 (t, J = 7.1 Hz, 3 H).
13 C NMR (75 MHz, CDCl3 ): δ = 209.6, 172.3, 147.6, 146.5, 132.3, 120.5, 108.6, 107.9, 101.2, 60.6, 53.8,
40.3, 33.7, 31.1, 21.0 14.3.
HRMS (DART): m /z [M + H]+ calcd for C17 H20 O5 : 305.13890; found: 305.14026.
2-(Benzo[d ][1,3]dioxol-5-yl)-6-(2-hydroxyethyl)cyclohexan-1-ol (20)
2-(Benzo[d ][1,3]dioxol-5-yl)-6-(2-hydroxyethyl)cyclohexan-1-ol (20)
To a solution of ester 19 (350 mg, 1.15 mmol) in THF (2.3 mL) at 0 °C was added LiAlH4 (261.9 mg, 6.9 mmol). After 10 min at 0 °C, the reaction mixture was allowed to warm
to r.t. and stirring was continued until starting material was totally consumed (TLC).
The reaction was quenched by careful addition of 10% aqueous NaOH solution until pH
7. The mixture was filtered over Celite and extracted with EtOAc (3 × 15 mL). The
combined organic layers were dried over Na2 SO4 , filtered, and concentrated in vacuo . Purification by column chromatography (silica gel, hexane/EtOAc, 15:1) afforded
compound 20 (colorless oil) as a separable mixture of diastereoisomers; combined yield: 263.4
mg (88%).
Diastereoisomer I
1 H NMR (500 MHz, CDCl3 ): δ = 6.77 (d, J = 7.9 Hz, 1 H), 6.75 (d, J = 1.6 Hz, 1 H), 6.70 (dd, J = 1.6, 7.9 Hz, 1 H), 5.94 (s, 2 H), 3.79–3.73 (m, 1 H), 3.68–3.59 (m, 1 H), 3.30
(t, J = 9.7 Hz, 1 H), 2.42 (ddd, J = 3.5, 10.0, 13.2 Hz, 1 H), 1.89–1.72 (m, 4 H), 1.67–1.51 (m, 4 H), 1.49–1.37 (m,
2 H).
13 C NMR (126 MHz, CDCl3 ): δ = 148.3, 146.7, 136.8, 121.3, 108.6, 107.9, 101.1, 78.8, 62.0, 52.6, 43.2, 38.5,
33.6, 33.1, 25.9.
Diastereoisomer II
1 H NMR (500 MHz, CDCl3 ): δ = 6.79–6.76 (m, 2 H), 6.71 (dd, J = 1.4, 8.0 Hz, 1 H), 5.93 (s, 2 H), 3.84 (s, 1 H), 3.78–3.66 (m, 2 H), 2.68 (dt,
J = 2.8, 12.9 Hz, 1 H), 1.95–1.86 (m, 2 H), 1.80–1.71 (m, 3 H), 1.68–1.57 (m, 3 H),
1.48–1.46 (m, 1 H).
13 C NMR (126 MHz, CDCl3 ): δ = 147.9, 146.3, 137.9, 120.6, 108.5, 108.4, 101.0, 73.5, 60.4, 48.6, 39.7, 36.4,
26.1, 25.6, 24.6.
HRMS (DART): m /z [M + H]+ calcd for C15 H20 O4 : 265.14398; found: 265.14490.
2-(3-(Benzo[d ][1,3]dioxol-5-yl)-2-oxocyclohexyl)acetaldehyde (21)
To a solution of oxalyl chloride (0.39 mL, 4.6 mmol) in DCM (5.8 mL) at –78 °C was
added DMSO (0.65 mL, 9.2 mmol) and the resulting solution was stirred for 30 min.
To this solution was transferred, via cannula, a solution of diol 20 (278 mg, 1.05 mmol) in DCM (5.8 mL). After 90 min at –78 °C, NEt3 (2.6 mL, 18.4 mmol) was added and the mixture stirred for a further 30 min. The reaction
mixture was allowed to warm to r.t. and concentrated under reduced pressure. The residue
was partitioned between H2 O and DCM and extracted with DCM (3 × 20 mL). The organic phase was dried (Na2 SO4 ), filtered, and concentrated in vacuo . After column chromatography (silica gel, hexane/EtOAc, 15:1), 21 was obtained as a separable mixture of diastereoisomers (2:1); combined yield: 238
mg (87%).
Diastereoisomer I
1 H NMR (300 MHz, CDCl3 ): δ = 9.77 (t, J = 0.97 Hz, 1 H), 6.80 (d, J = 8.0 Hz, 1 H), 6.76 (m, 1 H), 6.70 (ddd, J = 1.0, 1.8, 8.0 Hz, 1 H), 5.95 (s, 2 H), 3.77–3.70 (m, 1 H), 3.15–3.03 (m, 1 H),
2.92 (ddd, J = 1.1, 7.6, 17.6 Hz, 1 H), 2.58–2.47 (m, 1 H), 2.30 (ddd, J = 0.9, 5.2, 17.7 Hz, 1 H), 2.11–1.88 (m, 4 H), 1.81–1.71 (m, 1 H).
13 C NMR (75 MHz, CDCl3 ): δ = 209.40, 200.77, 147.66, 146.63, 132.16, 121.78, 109.22, 108.23, 101.04, 57.26,
46.03, 43.82, 36.44, 34.88, 25.67.
HRMS (DART): m /z [M + H]+ calcd for C15 H16 O4 : 261.11268; found: 261.11368.
Diastereoisomer II
1 H NMR (300 MHz, CDCl3 ): δ = 9.79 (t, J = 1.0 Hz, 1 H), 6.76 (d, J = 7.9 Hz, 1 H), 6.63 (d, J = 1.7 Hz, 1 H), 6.56 (ddd, J = 0.3, 1.7, 7.9 Hz, 1 H), 5.92 (s, 2 H), 3.68–3.58 (m, 1 H), 3.19–3.07 (m, 1 H),
2.99 (dd, J = 1.1, 7.6 Hz, 1 H), 2.30 (ddd, J = 0.8, 4.9, 17.6 Hz, 1 H), 2.35–2.17 (m, 2 H), 2.06–1.87 (m, 4 H).
7-(Benzo[d ][1,3]dioxol-5-yl)-4,5,6,7-tetrahydro-1H -indole (22)
7-(Benzo[d ][1,3]dioxol-5-yl)-4,5,6,7-tetrahydro-1H -indole (22)
To a solution of 21 (50 mg, 0.19 mmol) in MeOH (4 mL) at 0 °C were added ammonium acetate (30 mg, 0.38
mmol) and 4 Å molecular sieves (50 mg). After stirring for 2 h at 0 °C, MeOH was evaporated
off from the reaction flask and the residue was partitioned between H2 O and EtOAc. The mixture was extracted with EtOAc (3 × 10 mL), dried (Na2 SO4 ), filtered, and concentrated in vacuo . After column chromatography (silica gel, hexane/EtOAc, 15:1), 22 was obtained as a reddish oil; yield: 37 mg (80%).
1 H NMR (300 MHz, CDCl3 ): δ = 7.51 (br s, 1 H), 6.74 (d, J = 8.5 Hz, 1 H), 6.65–6.60 (m, 3 H), 6.01 (t, J = 2.6 Hz, 1 H), 5.92 (s, 2 H), 3.91 (t, J = 6.7 Hz, 1 H), 2.63–2.59 (m, 2 H), 2.18–2.10 (m, 1 H), 1.95–1.85 (m, 1 H), 1.78–1.67
(m, 2 H).
13 C NMR (75 MHz, CDCl3 ): δ = 147.9, 146.3, 139.2, 129.0, 121.2, 118.4, 116.5, 108.6, 108.5, 107.2, 101.0,
41.5, 34.5, 23.1, 22.7.
HRMS (DART): m /z [M + H]+ calcd for C15 H15 NO2 : 242.11810; found: 242.11773.
2-(3-(Benzo[d ][1,3]dioxol-5-yl)-2-oxocyclohexyl)acetonitrile (23)
2-(3-(Benzo[d ][1,3]dioxol-5-yl)-2-oxocyclohexyl)acetonitrile (23)
A solution of enol acetate 10 (150 mg, 0.58 mmol) and iodoacetonitrile (83 μL, 1.15 mmol) in 1,2-DCE (5.8 mL) was
refluxed under nitrogen for 10 min. Then, DLP was added portionwise (69.4 mg, 0.174
mmol) every hour until the complete consumption of starting material (monitored by
TLC). The reaction mixture was concentrated under reduced pressure and the residue
purified by column chromatography (silica gel, hexane/EtOAc/NEt3 , 20:1:0.1) to afford compound 23 as a mixture of diastereoisomers (24:1); combined yield: 124 mg (84%).
cis -Diastereoisomer 23a
White solid; mp 106–109 °C.
1 H NMR (300 MHz, CDCl3 ): δ = 6.77 (d, J = 7.9 Hz, 1 H), 6.62 (d, J = 1.7 Hz, 1 H), 6.56 (dd, J = 1.7, 7.9 Hz, 1 H), 5.93 (s, 2 H), 3.58 (dd, J = 5.4, 12.3 Hz, 1 H), 2.88–2.80 (m, 1 H), 2.71 (dd, J = 5.0, 17.1 Hz, 1 H), 2.49 (ddd, J = 2.4, 4.2, 9.9 Hz, 1 H), 2.43 (dd, J = 8.2, 17.1 Hz, 1 H), 2.34–2.29 (m, 1 H), 2.10–2.04 (m, 1 H), 1.97–1.91 (m, 2 H),
1.68–1.58 (m, 1 H).
13 C NMR (75 MHz, CDCl3 ): δ = 207.4, 147.8, 146.8, 131.4, 121.8, 118.6, 109.1, 108.3, 101.1, 57.2, 47.4,
36.3, 34.3, 25.2, 18.1.
HRMS (DART): m /z [M + H]+ calcd for C15 H15 NO3 : 258.11302; found: 258.11413.
trans -Diastereoisomer 23b
trans -Diastereoisomer 23b
Yellowish solid; mp 110–113 °C.
1 H NMR (300 MHz, CDCl3 ): δ = 6.79 (d, J = 8.0 Hz, 1 H), 6.73–6.70 (m, 1 H), 6.69–6.64 (m, 1 H), 5.97–5.94 (m, 2 H), 3.80–3.75
(m, 1 H), 2.79 (ddt, J = 5.3, 7.9, 10.6 Hz, 1 H), 2.64 (dd, J = 17.0, 5.1 Hz, 1 H), 2.60–2.52 (m, 1 H), 2.39 (dd, J = 8.1, 17.0 Hz, 1 H), 2.34–2.23 (m, 1 H), 2.11–1.91 (m, 2 H), 1.89–1.80 (m, 1 H),
1.67–1.52 (m, 1 H).
13 C NMR (75 MHz, CDCl3 ): δ = 209.4, 148.5, 146.8, 130.4, 120.2, 118.5, 108.9, 107.5, 101.3, 53.4, 43.7,
33.3, 30.1, 20.8, 18.1.
(3aR *,7S *,7aS *)-7-(Benzo[d ][1,3]dioxol-5-yl)octahydro-1H -indole (24)
(3aR *,7S *,7aS *)-7-(Benzo[d ][1,3]dioxol-5-yl)octahydro-1H -indole (24)
A mixture of nitrile 23a (66 mg, 0.26 mmol, 1 equiv) and PtO2 (19.8 mg, 30% w/w) in glacial acetic acid (3 mL) in a Fisher–Porter apparatus was
stirred under hydrogen atmosphere (250 psi) at r.t. for 24 h. A solution of 2 M NaOH
(20 mL) was added and the reaction mixture was extracted with DCM (3 × 20 mL). The
organic layer was washed with brine, dried over Na2 SO4 , filtered, and concentrated. The crude residue was purified by column chromatography
(silica gel, CHCl3 / MeOH/NEt3 , 9:1:0.1) to afford compound 24 as a yellow oil; yield: 56.6 mg (90%).
1 H NMR (300 MHz, CDCl3 ): δ = 6.82 (s, 1 H), 6.74 (brs, 2 H), 5.91 (s, 2 H), 3.23 (t, J = 3.8 Hz, 1 H), 3.17–3.08 (m, 1 H), 2.93–2.86 (m, 2 H), 2.12–2.05 (m, 1 H), 1.98–1.85
(m, 3 H), 1.65–1.50 (m, 3 H), 1.34–1.25 (m, 3 H).
13 C NMR (75 MHz, CDCl3 ): δ = 147.7, 146.0, 138.4, 120.4, 108.3, 108.2, 100.9, 63.6, 44.3, 43.3, 38.9, 31.3,
27.4, 25.8, 24.9.
HRMS (DART): m /z [M + H]+ calcd for C15 H19 NO2 : 246.14940; found: 246.14854.
