Planta Med 2005; 71(7): 599-603
DOI: 10.1055/s-2005-871263
Original Paper
Pharmacology
© Georg Thieme Verlag KG Stuttgart · New York

Inhibition of PDGF beta-Receptor Tyrosine Phosphorylation and its Downstream Intracellular Signal Transduction in Rat Aortic Vascular Smooth Muscle Cells by Kaempferol

Soo-Yeon Kim1 , Yong-Ri Jin1 , Yong Lim1 , Jin-Ho Kim1 , Mi-Ra Cho1 , Jin-Tae Hong1 , Hwan-Soo Yoo1 , Yeo-Pyo Yun1 , 2
  • 1College of Pharmacy, Chungbuk National University, Cheongju, Chungbuk, Korea
  • 2Research Center for Bioresource and Health, Chungbuk National University, Cheongju, Chungbuk, Korea
Further Information

Publication History

Received: September 20, 2004

Accepted: February 14, 2005

Publication Date:
18 July 2005 (online)

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Abstract

Kaempferol, a flavonoid present in human diet and plants, has been known to show cardiovascular protection via its anti-oxidant activity. In this study, we have investigated the effect of kaempferol on the proliferation of primary cultured rat aortic vascular smooth muscle cells (VSMCs). Kaempferol significantly inhibited 50 ng/mL platelet-derived growth factor (PDGF)-BB-induced proliferation and [3 H]-thymidine incorporation into DNA at concentrations of 5, 20 and 50 µM without any cytotoxicity. Kaempferol also inhibited the c-fos mRNA expression induced by PDGF-BB concentration-dependently. In addition, consistent with the inhibition of cell proliferation and c-fos mRNA expression, kaempferol inhibited the PDGF beta-receptor (Rβ) phosphorylation in a concentration-dependent manner. Accordingly, the downstream signal transductions of PDGF-Rβ such as ERK1/2, Akt and PLC-γ1 phosphorylation were also inhibited by kaempferol in the same pattern. These findings suggest that, in addition to its anti-oxidant activity, the cardiovascular protective effect of kaempferol may be mediated, at least in part, by the suppression of VSMC proliferation, which is due to the inhibition of PDGF-Rβ tyrosine phosphorylation and its downstream intracellular signal transduction.

References

Prof. Yeo-Pyo Yun

College of Pharmacy

Chungbuk National University

12 Gaeshin-Dong

Heungduk-Gu

Cheongju 361-763

Korea

Phone: +82-43-261-2821

Fax: +82-43-268-2732

Email: ypyun@chungbuk.ac.kr