Planta Med 2007; 73(12): 1327-1329
DOI: 10.1055/s-2007-981616
Physiology and Biotechnology
Letter
© Georg Thieme Verlag KG Stuttgart · New York

Biosynthesis of Polyacetylenes in Ambrosia maritima Hairy Roots

Sameh AbouZid1 , Yutaka Orihara2
  • 1Department of Pharmacognosy, Faculty of Pharmacy, Beni-Sueif University, Beni-Sueif, Egypt
  • 2Graduate School of Pharmaceutical Sciences, The University of Tokyo, Bunkyo-ku, Tokyo, Japan
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Publikationsverlauf

Received: March 30, 2007 Revised: June 27, 2007

Accepted: July 30, 2007

Publikationsdatum:
20. September 2007 (online)

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Abstract

Thiophene A and thiophene A diol are the major polyacetylenes isolated from the hairy roots of Ambrosia maritima (Asteraceae) cultured under continuous light irradiation. The biosynthesis of thiophene A was studied using [1 - 13C]-, [2 - 13C]-, [1,2 - 13C]-acetates. The biosynthesis of thiarubrine A, produced by hairy roots cultured in the dark, was studied using [1,2 - 13C]-acetates and [18 - 13C]-linoleic acid. Our results suggest a catabolic pathway for polyacetylenes biosynthesis from linoleic acid in hairy roots of Ambrosia maritima.

Abbreviations

DEPT:distortionless enhancement of polarization transfer

HMBC:heteronuclear multiple correlation

MeJ:methyl jasmonate

MS:Murashige and Skoog medium

References

Dr. Sameh AbouZid

Department of Pharmacognosy

Faculty of Pharmacy

Beni-Sueif University

Beni-Sueif

Egypt

Telefon: +20-2-335-9438

Fax: +20-82-231-7958

eMail: wssmd3000@yahoo.com