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Planta Med 2013; 79(14): 1307-1312
DOI: 10.1055/s-0033-1350732
Biological and Pharmacological Activity
Original Papers
Georg Thieme Verlag KG Stuttgart · New York

In Vitro Evaluation of Schistosomicidal Activity of Essential Oil of Mentha x villosa and Some of Its Chemical Constituents in Adult Worms of Schistosoma mansoni

Thiago José Matos-Rocha
1   Molecular and Cell Biology Laboratory, Department of Parasitology, Aggeu Magalhães Research Center, Oswaldo Cruz Foundation, Recife, Pernambuco, Brazil
2   Keizo Asami Immunopathology Laboratory, Federal University of Pernambuco, Recife, Pernambuco, Brazil
,
Marília Gabriela dos Santos Cavalcanti
1   Molecular and Cell Biology Laboratory, Department of Parasitology, Aggeu Magalhães Research Center, Oswaldo Cruz Foundation, Recife, Pernambuco, Brazil
2   Keizo Asami Immunopathology Laboratory, Federal University of Pernambuco, Recife, Pernambuco, Brazil
,
José Maria Barbosa-Filho
3   Federal University of Paraíba, João Pessoa, Paraíba, Brazil
,
Ana Silvia Suassuna Carneiro Lúcio
3   Federal University of Paraíba, João Pessoa, Paraíba, Brazil
,
Dyana Leal Veras
1   Molecular and Cell Biology Laboratory, Department of Parasitology, Aggeu Magalhães Research Center, Oswaldo Cruz Foundation, Recife, Pernambuco, Brazil
2   Keizo Asami Immunopathology Laboratory, Federal University of Pernambuco, Recife, Pernambuco, Brazil
,
Ana Paula Sampaio Feitosa
1   Molecular and Cell Biology Laboratory, Department of Parasitology, Aggeu Magalhães Research Center, Oswaldo Cruz Foundation, Recife, Pernambuco, Brazil
2   Keizo Asami Immunopathology Laboratory, Federal University of Pernambuco, Recife, Pernambuco, Brazil
,
José Pinto de Siqueira Júnior
3   Federal University of Paraíba, João Pessoa, Paraíba, Brazil
,
Reinaldo Nóbrega de Almeida
3   Federal University of Paraíba, João Pessoa, Paraíba, Brazil
,
Márcia Ortiz Mayo Marques
4   Natural Products Laboratory, Research and Development Center for Plant and Phytochemical Genetic Resources, Campinas Agronomical Institute, Campinas, São Paulo, Brazil
,
Luiz Carlos Alves
1   Molecular and Cell Biology Laboratory, Department of Parasitology, Aggeu Magalhães Research Center, Oswaldo Cruz Foundation, Recife, Pernambuco, Brazil
2   Keizo Asami Immunopathology Laboratory, Federal University of Pernambuco, Recife, Pernambuco, Brazil
,
Fábio André Brayner
1   Molecular and Cell Biology Laboratory, Department of Parasitology, Aggeu Magalhães Research Center, Oswaldo Cruz Foundation, Recife, Pernambuco, Brazil
2   Keizo Asami Immunopathology Laboratory, Federal University of Pernambuco, Recife, Pernambuco, Brazil
› Author Affiliations
Further Information

Publication History

received 26 June 2013
revised 03 July 2013

accepted 08 July 2013

Publication Date:
14 August 2013 (online)

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Abstract

This study aimed to determine the composition of the essential oil of Mentha x villosa and to evaluate its biological effects in vitro on adult worms of S. mansoni. Rotundifolone (70.96 %), limonene (8.75 %), trans-caryophyllene (1.46 %), and β-pinene (0.81 %) were shown to be the major constituents of this oil. Adult worms of S. mansoni were incubated with different concentrations of the essential oil (1, 10, 100, 250, 500, and 1000 µg/mL) and of its constituents rotundifolone (0.7, 3.54, 7.09, 70.96, 177.4, 354.8, and 700.96 µg/mL), limonene (43.75 µg/mL), trans-caryophyllene (7.3 µg/mL), and β-pinene (4.03 µg/mL). No schistosomicidal activity was identified at the trans-caryophyllene and β-pinene concentrations studied. However, use of the essential oil (10 µg/mL), rotundifolone (7.09 µg/mL), and limonene (43.75 µg/mL) resulted in decreased worm motility continuing until 96 hours of observation. At higher concentrations (100 and 70.96 µg/mL, respectively), both the essential oil and rotundifolone caused mortality among adult worms of S. mansoni. The positive control praziquantel caused the death of all parasites after 24 h of evaluation. The results from this study suggest that the essential oil of Mentha x villosa presents schistosomicidal efficacy.

Key words

Lamiaceae - Mentha x villosa - Schistosoma mansoni - schistosomicidal activity - natural products
 

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