Planta Med 1995; 61(5): 481-482
DOI: 10.1055/s-2006-958144
Letter

© Georg Thieme Verlag Stuttgart · New York

Enhanced Xanthotoxin Content in Regenerating Cultures of Ammi majus and Micropropagation

Madhumati Purohit, Deepshikha Pande, Aniruddha Datta, Prem S. Srivastava
  • Tissue Culture Laboratory, Department of Environmental Botany, Faculty of Science, Hamdard University, Hamdard Nagar, New Delhi 110062, India
Further Information

Publication History

1994

1995

Publication Date:
04 January 2007 (online)

Abstract

Since in Ammi majus the seed set and germination is poor attempts were made to propagate the plants through tissue cultures and monitor the yield of xanthotoxin (furanocoumarin) in the resultant callus and plantlets. The callus from cotyledonary leaves obtained on MS medium supplemented with α-indoleacetic acid (IAA) + kinetin (KN) + casein hydrolysate (CH), differentiated shoots in 100 % cultures when adenine was added to the above medium. Plantlets resulted on MS medium with indolebutyric acid (IBA) and glutamine. The yield of xanthotoxin in regenerating cultures was more than hitherto reported from any other tissue including the seeds. Isozymes could be used as reliable markers for the characterization of various stages of growth of callus and regenerants.